Comparison of holotomographic microscopy and coherence-controlled holographic microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00598790" target="_blank" >RIV/61388971:_____/24:00598790 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/24:10487415

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1111/jmi.13260" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/jmi.13260</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jmi.13260" target="_blank" >10.1111/jmi.13260</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of holotomographic microscopy and coherence-controlled holographic microscopy

Popis výsledku v původním jazyce

Quantitative phase imaging (QPI) is a powerful tool for label-free visualisation of living cells. Here, we compare two QPI microscopes - the Telight Q-Phase microscope and the Nanolive 3D Cell Explorer-fluo microscope. Both systems provide unbiased information about cell morphology, such as individual cell dry mass, perimeter and area. The Q-Phase microscope uses artefact-free, coherence-controlled holographic imaging technology to visualise cells in real time with minimal phototoxicity. The 3D Cell Explorer-fluo employs laser-based holotomography to reconstruct 3D images of living cells, visualising their internal structures and dynamics. Here, we analysed the strengths and limitations of both microscopes when examining two morphologically distinct cell lines - the cuboidal epithelial MDCK cells which form multicellular clusters and solitary growing Rat2 fibroblasts. We focus mainly on the ability of the devices to generate images suitable for single-cell segmentation by the built-in software, and we discuss the segmentation results and quantitative data generated from the segmented images. We show that both microscopes offer slightly different advantages, and the choice between them depends on the specific requirements and goals of the user.nLabel-free imaging techniques have revolutionised how we study biological samples, allowing for in-depth analysis without staining or labelling. In this context, quantitative phase imaging (QPI) or Quantitative Phase Microscopy (QPM) has emerged as a powerful tool. Unlike traditional phase-contrast microscopy, which provides qualitative insights, QPI offers quantitative data with information about cell dry mass or cell volume. Here, we compare two advanced QPI microscopy systems: the Telight Q-Phase microscope and the Nanolive 3D Cell Explorer-fluo microscope for visualisation of two morphologically distinct cell lines. Each of these instruments comes with its strengths and limitations and different software solutions for detailed cell analysis.

Název v anglickém jazyce

Comparison of holotomographic microscopy and coherence-controlled holographic microscopy

Popis výsledku anglicky

Quantitative phase imaging (QPI) is a powerful tool for label-free visualisation of living cells. Here, we compare two QPI microscopes - the Telight Q-Phase microscope and the Nanolive 3D Cell Explorer-fluo microscope. Both systems provide unbiased information about cell morphology, such as individual cell dry mass, perimeter and area. The Q-Phase microscope uses artefact-free, coherence-controlled holographic imaging technology to visualise cells in real time with minimal phototoxicity. The 3D Cell Explorer-fluo employs laser-based holotomography to reconstruct 3D images of living cells, visualising their internal structures and dynamics. Here, we analysed the strengths and limitations of both microscopes when examining two morphologically distinct cell lines - the cuboidal epithelial MDCK cells which form multicellular clusters and solitary growing Rat2 fibroblasts. We focus mainly on the ability of the devices to generate images suitable for single-cell segmentation by the built-in software, and we discuss the segmentation results and quantitative data generated from the segmented images. We show that both microscopes offer slightly different advantages, and the choice between them depends on the specific requirements and goals of the user.nLabel-free imaging techniques have revolutionised how we study biological samples, allowing for in-depth analysis without staining or labelling. In this context, quantitative phase imaging (QPI) or Quantitative Phase Microscopy (QPM) has emerged as a powerful tool. Unlike traditional phase-contrast microscopy, which provides qualitative insights, QPI offers quantitative data with information about cell dry mass or cell volume. Here, we compare two advanced QPI microscopy systems: the Telight Q-Phase microscope and the Nanolive 3D Cell Explorer-fluo microscope for visualisation of two morphologically distinct cell lines. Each of these instruments comes with its strengths and limitations and different software solutions for detailed cell analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microscopy

ISSN

0022-2720

e-ISSN

1365-2818

Svazek periodika

294

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

5-13

Kód UT WoS článku

001295203200001

EID výsledku v databázi Scopus

2-s2.0-85181914124