Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00636150" target="_blank" >RIV/61388971:_____/25:00636150 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/25:43909938

Výsledek na webu

<a href="https://www.nature.com/articles/s41564-025-02016-5" target="_blank" >https://www.nature.com/articles/s41564-025-02016-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41564-025-02016-5" target="_blank" >10.1038/s41564-025-02016-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea

Popis výsledku v původním jazyce

Aquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we show light energy transfer from diverse hydroxylated carotenoids to heimdallarchaeial rhodopsins (HeimdallRs) from uncultured marine planktonic members of 'Candidatus Kariarchaeaceae' ('Candidatus Asgardarchaeota'). These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light-absorbing retinal chromophore within HeimdallRs, enabling the use of light that is otherwise unavailable to the rhodopsin. Furthermore, we show elevated proton pumping by the antennas in HeimdallRs under white-light illumination, which better simulates the light conditions encountered by these archaea in their natural habitats. Our results indicate that light-harvesting antennas in microbial rhodopsins exist in families beyond xanthorhodopsins and proteorhodopsins and are present in both marine bacteria and archaea.

Název v anglickém jazyce

Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea

Popis výsledku anglicky

Aquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we show light energy transfer from diverse hydroxylated carotenoids to heimdallarchaeial rhodopsins (HeimdallRs) from uncultured marine planktonic members of 'Candidatus Kariarchaeaceae' ('Candidatus Asgardarchaeota'). These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light-absorbing retinal chromophore within HeimdallRs, enabling the use of light that is otherwise unavailable to the rhodopsin. Furthermore, we show elevated proton pumping by the antennas in HeimdallRs under white-light illumination, which better simulates the light conditions encountered by these archaea in their natural habitats. Our results indicate that light-harvesting antennas in microbial rhodopsins exist in families beyond xanthorhodopsins and proteorhodopsins and are present in both marine bacteria and archaea.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/EH22_008%2F0004624" target="_blank" >EH22_008/0004624: PHOTOMACHINES-Reorganizace fotosyntetických buněk za účelem vysoké produkce terapeutických peptidů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Microbiology

ISSN

2058-5276

e-ISSN

2058-5276

Svazek periodika

10

Číslo periodika v rámci svazku

June 25

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

1484-1500

Kód UT WoS článku

001498534300001

EID výsledku v databázi Scopus

2-s2.0-105006810440