Selective Choice of the Efficient Carotenoid Antenna by a Xanthorhodopsin: Controlling Factors for Binding and Excitation Energy Transfer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00637891" target="_blank" >RIV/61388971:_____/25:00637891 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/jacsau.4c01243" target="_blank" >https://pubs.acs.org/doi/10.1021/jacsau.4c01243</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/jacsau.4c01243" target="_blank" >10.1021/jacsau.4c01243</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Selective Choice of the Efficient Carotenoid Antenna by a Xanthorhodopsin: Controlling Factors for Binding and Excitation Energy Transfer

Popis výsledku v původním jazyce

Despite extensive research on carotenoids and microbial rhodopsins in aquatic environments, a fundamental understanding of the binding requirements of carotenoids that serve as auxiliary light-harvesting antennas for rhodopsins is still lacking. Our recent discovery of 3-hydroxylated xanthophyll-binding proteorhodopsins and xanthorhodopsins prompted us to investigate the role of keto and hydroxy functional groups in carotenoid binding to rhodopsins and their influence on energy transfer to the retinal chromophore. In this study, we examined the binding of 12 carotenoids to rhodopsin Kin4B8 (a protein of the xanthorhodopsin family, GenBank: OP056329) and assessed the energy transfer between the carotenoid and the retinal chromophore. We found that 3-hydroxylated xanthophylls were the most effective light-harvesting antennas among the carotenoids studied. While 4-ketocarotenoids also bound to the protein, their energy transfer efficiency was significantly reduced. In contrast, the presence of a 4-hydroxy group or the substitution of the beta-ionone ring by an epsilon-ionone ring completely prevented binding. Furthermore, mutagenesis studies of Kin4B8 suggest that specific residues play a key role in the selective binding of carotenoids. These findings provide valuable insights into the structural determinants of rhodopsin-carotenoid interactions, which may aid in predicting the recruitment of various carotenoid antennas by retinal proteins.

Název v anglickém jazyce

Selective Choice of the Efficient Carotenoid Antenna by a Xanthorhodopsin: Controlling Factors for Binding and Excitation Energy Transfer

Popis výsledku anglicky

Despite extensive research on carotenoids and microbial rhodopsins in aquatic environments, a fundamental understanding of the binding requirements of carotenoids that serve as auxiliary light-harvesting antennas for rhodopsins is still lacking. Our recent discovery of 3-hydroxylated xanthophyll-binding proteorhodopsins and xanthorhodopsins prompted us to investigate the role of keto and hydroxy functional groups in carotenoid binding to rhodopsins and their influence on energy transfer to the retinal chromophore. In this study, we examined the binding of 12 carotenoids to rhodopsin Kin4B8 (a protein of the xanthorhodopsin family, GenBank: OP056329) and assessed the energy transfer between the carotenoid and the retinal chromophore. We found that 3-hydroxylated xanthophylls were the most effective light-harvesting antennas among the carotenoids studied. While 4-ketocarotenoids also bound to the protein, their energy transfer efficiency was significantly reduced. In contrast, the presence of a 4-hydroxy group or the substitution of the beta-ionone ring by an epsilon-ionone ring completely prevented binding. Furthermore, mutagenesis studies of Kin4B8 suggest that specific residues play a key role in the selective binding of carotenoids. These findings provide valuable insights into the structural determinants of rhodopsin-carotenoid interactions, which may aid in predicting the recruitment of various carotenoid antennas by retinal proteins.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA25-16833S" target="_blank" >GA25-16833S: Duální fototrofie (DualPhoto)</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JACS Au

ISSN

2691-3704

e-ISSN

2691-3704

Svazek periodika

5

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

3070-3081

Kód UT WoS článku

001518551400001

EID výsledku v databázi Scopus

2-s2.0-105009339994