Untargeted Metabolomics and Antibacterial Properties of Streptomyces Species Sourced From Thai Mangrove

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00639118" target="_blank" >RIV/61388971:_____/25:00639118 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1155/sci5/5510017" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1155/sci5/5510017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/sci5/5510017" target="_blank" >10.1155/sci5/5510017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Untargeted Metabolomics and Antibacterial Properties of Streptomyces Species Sourced From Thai Mangrove

Popis výsledku v původním jazyce

Novel antimicrobial agents are urgently needed to combat the global threat of antimicrobial resistance. Actinobacteria are remarkable producers of bioactive compounds, which are crucial for the discovery of novel drugs to combat antimicrobial resistance. In the current study, bioactivity, phylogenetic analysis, and a dereplication approach were employed to quickly analyze the metabolomic profiles of selected strains and aid in selection strain prioritization. The aim of this study was to screen Thai actinobacteria isolated from the mangrove ecosystem for antibacterial activities. Taxonomic identification confirmed the classification of these isolates within the genus Streptomyces. Eleven strains identified as Streptomyces yogyakartensis, S. globisporus, S. albiaxialis, S. misionensis, S. iranensis, S. sanyensis, and S. diastaticus. The crude extracts of the selected actinobacteria strains exhibited antibacterial activities, with minimum inhibitory concentrations ranging from 9.9 to 1250 mu g/mL against methicillin-resistant Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae. Mass-guided molecular networking analysis of the crude extract revealed the metabolomic complexity of the isolates. Structurally unique compounds such as desferrioxamine E, elaiophylin, kanchanamycin C, bisacuberin, dehydroxynordicadamine, desmethylenylnocardamine, chymostatinol A, chymostatin B, desferrioxamine G, ferrioxamine E, and legonoxamine A were detected in the crude extracts. Notably, we report the potential of bioactive compounds isolated from Thai mangrove actinobacteria, demonstrating diverse biological activities. This study demonstrated the use of metabolomics to annotate putative bioactive compounds, prioritize strains, and discover novel bioactive metabolites.

Název v anglickém jazyce

Untargeted Metabolomics and Antibacterial Properties of Streptomyces Species Sourced From Thai Mangrove

Popis výsledku anglicky

Novel antimicrobial agents are urgently needed to combat the global threat of antimicrobial resistance. Actinobacteria are remarkable producers of bioactive compounds, which are crucial for the discovery of novel drugs to combat antimicrobial resistance. In the current study, bioactivity, phylogenetic analysis, and a dereplication approach were employed to quickly analyze the metabolomic profiles of selected strains and aid in selection strain prioritization. The aim of this study was to screen Thai actinobacteria isolated from the mangrove ecosystem for antibacterial activities. Taxonomic identification confirmed the classification of these isolates within the genus Streptomyces. Eleven strains identified as Streptomyces yogyakartensis, S. globisporus, S. albiaxialis, S. misionensis, S. iranensis, S. sanyensis, and S. diastaticus. The crude extracts of the selected actinobacteria strains exhibited antibacterial activities, with minimum inhibitory concentrations ranging from 9.9 to 1250 mu g/mL against methicillin-resistant Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae. Mass-guided molecular networking analysis of the crude extract revealed the metabolomic complexity of the isolates. Structurally unique compounds such as desferrioxamine E, elaiophylin, kanchanamycin C, bisacuberin, dehydroxynordicadamine, desmethylenylnocardamine, chymostatinol A, chymostatin B, desferrioxamine G, ferrioxamine E, and legonoxamine A were detected in the crude extracts. Notably, we report the potential of bioactive compounds isolated from Thai mangrove actinobacteria, demonstrating diverse biological activities. This study demonstrated the use of metabolomics to annotate putative bioactive compounds, prioritize strains, and discover novel bioactive metabolites.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientifica

ISSN

2090-908X

e-ISSN

2090-908X

Svazek periodika

2025

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

5510017

Kód UT WoS článku

001494937500001

EID výsledku v databázi Scopus

2-s2.0-105006746486