Ajmaline-induced block of sodium current in rat ventricular myocytes.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388998%3A_____%2F02%3A51020083" target="_blank" >RIV/61388998:_____/02:51020083 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/02:00007153

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Ajmaline-induced block of sodium current in rat ventricular myocytes.

Popis výsledku v původním jazyce

The aim of the present study was to explore concentration-, voltage- and frequency-dependence of the sodium current (INa) block induced by ajmaline in enzymatically isolated rat ventricular myocytes. Voltage clamp experiments in whole-cell patch-clamp arrangement were performed on 32 cells. The blocking effect was evaluated in the range of concentrations between 0.3 and 5000 mmol/l at room temperature. The level of block was concentration-dependent and the effect was fully reversible. Pooled data analysis (18 cells and 25 measurements) resulted in EC50 = 8.2 1.5 mmol/l and the Hill coefficient of 0.62 0.08. The voltage- and frequency-dependence of the drug effect was analysed at 3 mmol/l. The block appeared to be voltage-dependent but frequency-independent in contrast to propafenone (0.2 mmol/l). An analysis of the rate of block level variations in response to voltage steps revealed substantial differences between kinetics of the block development in both drugs that corresponded well w

Název v anglickém jazyce

Ajmaline-induced block of sodium current in rat ventricular myocytes.

Popis výsledku anglicky

The aim of the present study was to explore concentration-, voltage- and frequency-dependence of the sodium current (INa) block induced by ajmaline in enzymatically isolated rat ventricular myocytes. Voltage clamp experiments in whole-cell patch-clamp arrangement were performed on 32 cells. The blocking effect was evaluated in the range of concentrations between 0.3 and 5000 mmol/l at room temperature. The level of block was concentration-dependent and the effect was fully reversible. Pooled data analysis (18 cells and 25 measurements) resulted in EC50 = 8.2 1.5 mmol/l and the Hill coefficient of 0.62 0.08. The voltage- and frequency-dependence of the drug effect was analysed at 3 mmol/l. The block appeared to be voltage-dependent but frequency-independent in contrast to propafenone (0.2 mmol/l). An analysis of the rate of block level variations in response to voltage steps revealed substantial differences between kinetics of the block development in both drugs that corresponded well w

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scripta Medica

ISSN

1211-3395

e-ISSN

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Svazek periodika

75

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

169-178

Kód UT WoS článku

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EID výsledku v databázi Scopus

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