Glucose determination using a re-usable enzyme-modified ion track membrane sensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F09%3A00338731" target="_blank" >RIV/61389005:_____/09:00338731 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Glucose determination using a re-usable enzyme-modified ion track membrane sensor

Popis výsledku v původním jazyce

A novel concept for a glucose biosensor based on the enzyme glucose oxidase covalently linked to nanopores of etched nuclear track membranes is presented. This device can be used to detect physiologically relevant glucose concentrations between 10 mu M and 1 M. The sensitive catalytic sensor can be made re-usable due to the production of diffusible products from the oxidative biomolecular recognition event. We have demonstrated both the simplicity with which this sensor can be produced and the stabilityof the enzyme embedded in the nanopores. We strongly believe that the approach could be expanded for different enzymes and variable configurations of sensing and catalysis using membranes with nanopores for biocatalysis and enrichment of substrates andproducts. This is the first time that such devices are being used for biocatalytic transformations.

Název v anglickém jazyce

Glucose determination using a re-usable enzyme-modified ion track membrane sensor

Popis výsledku anglicky

A novel concept for a glucose biosensor based on the enzyme glucose oxidase covalently linked to nanopores of etched nuclear track membranes is presented. This device can be used to detect physiologically relevant glucose concentrations between 10 mu M and 1 M. The sensitive catalytic sensor can be made re-usable due to the production of diffusible products from the oxidative biomolecular recognition event. We have demonstrated both the simplicity with which this sensor can be produced and the stabilityof the enzyme embedded in the nanopores. We strongly believe that the approach could be expanded for different enzymes and variable configurations of sensing and catalysis using membranes with nanopores for biocatalysis and enrichment of substrates andproducts. This is the first time that such devices are being used for biocatalytic transformations.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BG - Jaderná, atomová a molekulová fyzika, urychlovače

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biosensors and Bioeletronics

ISSN

0956-5663

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000265811000064

EID výsledku v databázi Scopus

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