Coupled chemical reactions in dynamic nanometric confinement: VII. Biosensors based on swift heavy ion tracks with membranes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F17%3A00474575" target="_blank" >RIV/61389005:_____/17:00474575 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/10420150.2017.1290633" target="_blank" >http://dx.doi.org/10.1080/10420150.2017.1290633</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/10420150.2017.1290633" target="_blank" >10.1080/10420150.2017.1290633</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Coupled chemical reactions in dynamic nanometric confinement: VII. Biosensors based on swift heavy ion tracks with membranes

Popis výsledku v původním jazyce

In previous papers it was shown that the coupling of the two chemical reactions: {NaOH etchant - PET polymer} and {NaOH etchant - AgNO3 solution} within the dynamic confinement of etched swift heavy ion tracks eventually leads to the formation of tiny Ag2O membranes within these nanopores, thus separating the latter ones into two adjacent segments. It is shown here that the deposition of enzymes in these two segments transforms these structures into biosensors. In our earlier developed sensors with transparent etched ion tracks, we frequently used glucose oxidase as enzyme and glucose as analyte. In these cases, the enzymatic reaction within the tracks leads to a change in the pH value of the confined solution and hence also in the track conductivity, so these structures can be used for biosensing. When applying, for easy comparison, the same enzyme/ analyte combination to the segmented sensor arrangement presented here, we find a striking improvement in detection sensitivity which points at a different biosensing mechanism due to intrinsic polarisation effects across the newly inserted membranes.

Název v anglickém jazyce

Coupled chemical reactions in dynamic nanometric confinement: VII. Biosensors based on swift heavy ion tracks with membranes

Popis výsledku anglicky

In previous papers it was shown that the coupling of the two chemical reactions: {NaOH etchant - PET polymer} and {NaOH etchant - AgNO3 solution} within the dynamic confinement of etched swift heavy ion tracks eventually leads to the formation of tiny Ag2O membranes within these nanopores, thus separating the latter ones into two adjacent segments. It is shown here that the deposition of enzymes in these two segments transforms these structures into biosensors. In our earlier developed sensors with transparent etched ion tracks, we frequently used glucose oxidase as enzyme and glucose as analyte. In these cases, the enzymatic reaction within the tracks leads to a change in the pH value of the confined solution and hence also in the track conductivity, so these structures can be used for biosensing. When applying, for easy comparison, the same enzyme/ analyte combination to the segmented sensor arrangement presented here, we find a striking improvement in detection sensitivity which points at a different biosensing mechanism due to intrinsic polarisation effects across the newly inserted membranes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/GBP108%2F12%2FG108" target="_blank" >GBP108/12/G108: Příprava, modifikace a charakterizace materiálů zářením</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Radiation Effects and Defects in Solids

ISSN

1042-0150

e-ISSN

—

Svazek periodika

172

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

159-173

Kód UT WoS článku

000399481700018

EID výsledku v databázi Scopus

2-s2.0-85017588053