Radiation damage to DNA in DNA-protein complexes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F11%3A00361467" target="_blank" >RIV/61389005:_____/11:00361467 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.mrfmmm.2011.02.003" target="_blank" >http://dx.doi.org/10.1016/j.mrfmmm.2011.02.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mrfmmm.2011.02.003" target="_blank" >10.1016/j.mrfmmm.2011.02.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Radiation damage to DNA in DNA-protein complexes

Popis výsledku v původním jazyce

The most aggressive product of water radiolysis, the hydroxyl (OH) radical, is responsible for the indirect effect of ionizing radiations on DNA in solution and aerobic conditions. Monte-Carlo based model RADACK, allows calculating the relative probability of damage of each nucleotide of DNA irradiated alone or in complexes with proteins. The confrontation of the calculated values with the results of the radiolytic footprinting experiments together with molecular modeling calculations show that: (1) theextent and location of the lesions are strongly dependent on the structure of DNA, which in turns is modulated by the base sequence and by the binding of proteins and (2) the regions in contact with the protein can be protected against the attack by thehydroxyl radicals via masking of the binding site and by scavenging of the radicals.

Název v anglickém jazyce

Radiation damage to DNA in DNA-protein complexes

Popis výsledku anglicky

The most aggressive product of water radiolysis, the hydroxyl (OH) radical, is responsible for the indirect effect of ionizing radiations on DNA in solution and aerobic conditions. Monte-Carlo based model RADACK, allows calculating the relative probability of damage of each nucleotide of DNA irradiated alone or in complexes with proteins. The confrontation of the calculated values with the results of the radiolytic footprinting experiments together with molecular modeling calculations show that: (1) theextent and location of the lesions are strongly dependent on the structure of DNA, which in turns is modulated by the base sequence and by the binding of proteins and (2) the regions in contact with the protein can be protected against the attack by thehydroxyl radicals via masking of the binding site and by scavenging of the radicals.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

ISSN

0027-5107

e-ISSN

—

Svazek periodika

711

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

41-48

Kód UT WoS článku

000291914700005

EID výsledku v databázi Scopus

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