Micronuclei in human peripheral blood lymphocytes exposed to mixed beams of X-rays and alpha particles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F12%3A00380755" target="_blank" >RIV/61389005:_____/12:00380755 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00411-012-0417-x" target="_blank" >http://dx.doi.org/10.1007/s00411-012-0417-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00411-012-0417-x" target="_blank" >10.1007/s00411-012-0417-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Micronuclei in human peripheral blood lymphocytes exposed to mixed beams of X-rays and alpha particles

Popis výsledku v původním jazyce

The purpose of this study was to analyse the cytogenetic effect of exposing human peripheral blood lymphocytes (PBL) to a mixed beam of alpha particles and X-rays. Whole blood collected from one donor was exposed to different doses of alpha particles (Am-241), X-rays and a combination of both. Micronuclei (MN) in binucleated PBL were scored as the endpoint. Moreover, the size of MN was measured. The results show that exposure of PBL to a mixed beam of high and low linear energy transfer radiation led tosignificantly higher than expected frequencies of MN. The measurement of MN size did not reveal any differences between the effect of alpha particles and mixed beam. In conclusion, a combined exposure of PBL to alpha particles and X-rays leads to a synergistic effect as measured by the frequency of MN. From the analysis of MN distributions, we conclude that the increase was due to an impaired repair of X-ray-induced DNA damage.

Název v anglickém jazyce

Micronuclei in human peripheral blood lymphocytes exposed to mixed beams of X-rays and alpha particles

Popis výsledku anglicky

The purpose of this study was to analyse the cytogenetic effect of exposing human peripheral blood lymphocytes (PBL) to a mixed beam of alpha particles and X-rays. Whole blood collected from one donor was exposed to different doses of alpha particles (Am-241), X-rays and a combination of both. Micronuclei (MN) in binucleated PBL were scored as the endpoint. Moreover, the size of MN was measured. The results show that exposure of PBL to a mixed beam of high and low linear energy transfer radiation led tosignificantly higher than expected frequencies of MN. The measurement of MN size did not reveal any differences between the effect of alpha particles and mixed beam. In conclusion, a combined exposure of PBL to alpha particles and X-rays leads to a synergistic effect as measured by the frequency of MN. From the analysis of MN distributions, we conclude that the increase was due to an impaired repair of X-ray-induced DNA damage.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Radiation and Environmental Biophysics

ISSN

0301-634X

e-ISSN

—

Svazek periodika

51

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

283-293

Kód UT WoS článku

000306694000006

EID výsledku v databázi Scopus

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