Different cadmium tolerance of two isolates of Hebeloma mesophaeum showing different basal expression levels of metallothionein (HmMT3) gene

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F19%3A00503681" target="_blank" >RIV/61389005:_____/19:00503681 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985831:_____/19:00504081 RIV/60461373:22330/19:43919770

Výsledek na webu

<a href="https://doi.org/10.1016/j.funbio.2018.12.006" target="_blank" >https://doi.org/10.1016/j.funbio.2018.12.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.funbio.2018.12.006" target="_blank" >10.1016/j.funbio.2018.12.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Different cadmium tolerance of two isolates of Hebeloma mesophaeum showing different basal expression levels of metallothionein (HmMT3) gene

Popis výsledku v původním jazyce

Hebeloma mesophaeum is an ectomycorrhizal fungus frequently associated with metal disturbed environments. In this work, we examined Ag, Cd, and Zn tolerance of H. mesophaeum isolates from heavy metal-polluted (isolate Prib) and clean (isolate Rez) sites. Both mycelia showed essentially the same level of Ag and Zn tolerance, but Prib was more Cd tolerant. In short-term exposures, Prib accumulated slightly less Cd than Rez. Size exclusion chromatography of cell-free extracts and fluorescence microscopy of hyphae with a Cd-specific fluorescent tracer revealed that substantial proportion of Cd was contained in the vacuoles in both isolates. Considering that the proportion of Cd associated with fractions attributable to Cd complexes with cytosolic, metallothionein (MT) peptides was higher in Prib, we examined the copy number and basal levels of HmMTs genes in Rez and Prib. While no difference between the isolates was observed in the gene copy numbers and basal levels of HmMT1 transcripts, the basal transcription of HmMT3 was 3-fold higher in Prib. These observations suggest that MTs provide in Prib better protection against Cd. Furthermore, the higher Cd tolerance in Prib can be to some extent also supported by the efflux or reduced uptake of Cd in the hyphae.

Název v anglickém jazyce

Different cadmium tolerance of two isolates of Hebeloma mesophaeum showing different basal expression levels of metallothionein (HmMT3) gene

Popis výsledku anglicky

Hebeloma mesophaeum is an ectomycorrhizal fungus frequently associated with metal disturbed environments. In this work, we examined Ag, Cd, and Zn tolerance of H. mesophaeum isolates from heavy metal-polluted (isolate Prib) and clean (isolate Rez) sites. Both mycelia showed essentially the same level of Ag and Zn tolerance, but Prib was more Cd tolerant. In short-term exposures, Prib accumulated slightly less Cd than Rez. Size exclusion chromatography of cell-free extracts and fluorescence microscopy of hyphae with a Cd-specific fluorescent tracer revealed that substantial proportion of Cd was contained in the vacuoles in both isolates. Considering that the proportion of Cd associated with fractions attributable to Cd complexes with cytosolic, metallothionein (MT) peptides was higher in Prib, we examined the copy number and basal levels of HmMTs genes in Rez and Prib. While no difference between the isolates was observed in the gene copy numbers and basal levels of HmMT1 transcripts, the basal transcription of HmMT3 was 3-fold higher in Prib. These observations suggest that MTs provide in Prib better protection against Cd. Furthermore, the higher Cd tolerance in Prib can be to some extent also supported by the efflux or reduced uptake of Cd in the hyphae.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA16-15065S" target="_blank" >GA16-15065S: Faktory ovlivňující akumulaci těžkých kovů velkými houbami</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fungal Biology

ISSN

1878-6146

e-ISSN

—

Svazek periodika

123

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

247-254

Kód UT WoS článku

000461403100008

EID výsledku v databázi Scopus

2-s2.0-85059540159