Breaking the DNA by soft X-rays in the water window reveals the scavenging and temporal behaviour of ·OH radicals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F24%3A00602193" target="_blank" >RIV/61389005:_____/24:00602193 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/24:00602193

Výsledek na webu

<a href="https://hdl.handle.net/11104/0360172" target="_blank" >https://hdl.handle.net/11104/0360172</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-024-79328-9" target="_blank" >10.1038/s41598-024-79328-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Breaking the DNA by soft X-rays in the water window reveals the scavenging and temporal behaviour of ·OH radicals

Popis výsledku v původním jazyce

A laser-plasma source emitting photons with energies in the water window spectral range has been used to reveal the radiation chemical yields of single-strand breaks in plasmid DNA as a function of ·OH radical scavenger concentration. Direct and indirect effects were investigated separately using DNA samples with various levels of hydration. We experimentally determined the value of the efficiency factor for strand cleavage in DNA caused by the reaction with ·OH radicals at 0.11, which was previously found in the theoretical studies. Additionally, the radiation chemical yield of ·OH radicals specific to the water window radiation emission of the source was determined by comparison with the gamma radiation-induced strand break yields. The ·OH radical yield determined using the plasmid DNA samples as a model was similar to the yield found using sensitive fluorescent dosimeters in previous experiments.

Název v anglickém jazyce

Breaking the DNA by soft X-rays in the water window reveals the scavenging and temporal behaviour of ·OH radicals

Popis výsledku anglicky

A laser-plasma source emitting photons with energies in the water window spectral range has been used to reveal the radiation chemical yields of single-strand breaks in plasmid DNA as a function of ·OH radical scavenger concentration. Direct and indirect effects were investigated separately using DNA samples with various levels of hydration. We experimentally determined the value of the efficiency factor for strand cleavage in DNA caused by the reaction with ·OH radicals at 0.11, which was previously found in the theoretical studies. Additionally, the radiation chemical yield of ·OH radicals specific to the water window radiation emission of the source was determined by comparison with the gamma radiation-induced strand break yields. The ·OH radical yield determined using the plasmid DNA samples as a model was similar to the yield found using sensitive fluorescent dosimeters in previous experiments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

14

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

28515

Kód UT WoS článku

001359321100044

EID výsledku v databázi Scopus

2-s2.0-85209575100