Proton-induced direct and indirect damage of plasmid DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F15%3A00448614" target="_blank" >RIV/68378271:_____/15:00448614 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/15:00448614 RIV/68407700:21340/15:00235108

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00411-015-0605-6" target="_blank" >http://dx.doi.org/10.1007/s00411-015-0605-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00411-015-0605-6" target="_blank" >10.1007/s00411-015-0605-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proton-induced direct and indirect damage of plasmid DNA

Popis výsledku v původním jazyce

Clustered DNA damage induced by 10, 20 and 30 MeV protons in pBR322 plasmid DNA was investigated. Besides determination of strand breaks, additional lesions were detected using base excision repair enzymes. The plasmid was irradiated in dry form, where indirect radiation effects were almost fully suppressed, and in water solution containing only minimal residual radical scavenger. Simultaneous irradiation of the plasmid DNA in the dry form and in the solution demonstrated the contribution of the indirect effect as prevalent. The damage composition slightly differed when comparing the results for liquid and dry samples. The obtained data were also subjected to analysis concerning different methodological approaches, particularly the influence of irradiation geometry, models used for calculation of strand break yields and interpretation of the strand breaks detected with the enzymes. It was shown that these parameters strongly affect the results.

Název v anglickém jazyce

Proton-induced direct and indirect damage of plasmid DNA

Popis výsledku anglicky

Clustered DNA damage induced by 10, 20 and 30 MeV protons in pBR322 plasmid DNA was investigated. Besides determination of strand breaks, additional lesions were detected using base excision repair enzymes. The plasmid was irradiated in dry form, where indirect radiation effects were almost fully suppressed, and in water solution containing only minimal residual radical scavenger. Simultaneous irradiation of the plasmid DNA in the dry form and in the solution demonstrated the contribution of the indirect effect as prevalent. The damage composition slightly differed when comparing the results for liquid and dry samples. The obtained data were also subjected to analysis concerning different methodological approaches, particularly the influence of irradiation geometry, models used for calculation of strand break yields and interpretation of the strand breaks detected with the enzymes. It was shown that these parameters strongly affect the results.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Radiation and Environmental Biophysics

ISSN

0301-634X

e-ISSN

—

Svazek periodika

54

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

343-352

Kód UT WoS článku

000358328800010

EID výsledku v databázi Scopus

2-s2.0-84938996562