Impact of nanosilver on various DNA lesions and HPRT gene mutations - effects of charge and surface coating

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00446216" target="_blank" >RIV/61389013:_____/15:00446216 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/s12989-015-0100-x" target="_blank" >http://dx.doi.org/10.1186/s12989-015-0100-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12989-015-0100-x" target="_blank" >10.1186/s12989-015-0100-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impact of nanosilver on various DNA lesions and HPRT gene mutations - effects of charge and surface coating

Popis výsledku v původním jazyce

The main goal of this research was to study the interactions of a fully characterized set of silver nanomaterials (Ag ENMs) with cells in vitro, according to the standards of Good Laboratory Practices (GLP), to assure the quality of nanotoxicology research. We were interested in whether Ag ENMs synthesized by the same method, with the same size distribution, shape and specific surface area, but with different charges and surface compositions could give different biological responses. A range of methodsand toxicity endpoints were applied to study the impacts of interaction of the Ag ENMs with TK6 cells. As tests of viability, relative growth activity and trypan blue exclusion were applied. Genotoxicity was evaluated by the alkaline comet assay for detection of strand breaks and oxidized purines. The mutagenic potential of Ag ENMs was investigated with the in vitro HPRT gene mutation test on V79-4 cells according to the OECD protocol. Ag ENM agglomeration, dissolution as well as uptake

Název v anglickém jazyce

Impact of nanosilver on various DNA lesions and HPRT gene mutations - effects of charge and surface coating

Popis výsledku anglicky

The main goal of this research was to study the interactions of a fully characterized set of silver nanomaterials (Ag ENMs) with cells in vitro, according to the standards of Good Laboratory Practices (GLP), to assure the quality of nanotoxicology research. We were interested in whether Ag ENMs synthesized by the same method, with the same size distribution, shape and specific surface area, but with different charges and surface compositions could give different biological responses. A range of methodsand toxicity endpoints were applied to study the impacts of interaction of the Ag ENMs with TK6 cells. As tests of viability, relative growth activity and trypan blue exclusion were applied. Genotoxicity was evaluated by the alkaline comet assay for detection of strand breaks and oxidized purines. The mutagenic potential of Ag ENMs was investigated with the in vitro HPRT gene mutation test on V79-4 cells according to the OECD protocol. Ag ENM agglomeration, dissolution as well as uptake

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Particle and Fibre Toxicology

ISSN

1743-8977

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

24 July

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

20

Strana od-do

"25_1"-"25_20"

Kód UT WoS článku

000358406900001

EID výsledku v databázi Scopus

2-s2.0-84937797469