Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00463732" target="_blank" >RIV/61389013:_____/17:00463732 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.colsurfb.2016.10.007" target="_blank" >http://dx.doi.org/10.1016/j.colsurfb.2016.10.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2016.10.007" target="_blank" >10.1016/j.colsurfb.2016.10.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals

Popis výsledku v původním jazyce

Changes in cholesterol concentration in the plasma membrane of presynaptic nerve terminals non-specifically modulate glutamate transport and homeostasis in the central nervous system. Reduction of the cholesterol content in isolated rat brain nerve terminals (synaptosomes) using cholesterol depleting agents decreases the glutamate uptake and increases the extracellular level of glutamate in nerve terminals. Extraction of cholesterol from the plasma membrane and its further removal from the synaptosomes by external magnetic field can be achieved by means of magnetic nanoparticles with immobilized cholesterol-depleting agent such as O-methyl-beta-cyclodextrin (MCD). A simple approach is developed for preparation of maghemite (gamma-Fe2O3) nanoparticles containing chemically bonded MCD. The method is based on preparation of a silanization agent containing MCD. It is synthesized by the reaction of triethoxy(3-isocyanatopropyl)silane with MCD. Base-catalyzed silanization of superparamagnetic gamma-Fe2O3 provides a relatively stable colloid product containing 48 mu mol of MCD g(-1). MCD-modified gamma-Fe2O3 nanoparticles decrease the initial rate of the uptake and accumulation of L-[C-14]glutamate and increase the extracellular L-[C-14]glutamate level in the preparation of nerve terminals. The effect of MCD-immobilized nanoparticles is the same as that of MCD solution moreover, magnetic manipulation of the nanoparticles enables removal of bonded cholesterol.

Název v anglickém jazyce

Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals

Popis výsledku anglicky

Changes in cholesterol concentration in the plasma membrane of presynaptic nerve terminals non-specifically modulate glutamate transport and homeostasis in the central nervous system. Reduction of the cholesterol content in isolated rat brain nerve terminals (synaptosomes) using cholesterol depleting agents decreases the glutamate uptake and increases the extracellular level of glutamate in nerve terminals. Extraction of cholesterol from the plasma membrane and its further removal from the synaptosomes by external magnetic field can be achieved by means of magnetic nanoparticles with immobilized cholesterol-depleting agent such as O-methyl-beta-cyclodextrin (MCD). A simple approach is developed for preparation of maghemite (gamma-Fe2O3) nanoparticles containing chemically bonded MCD. The method is based on preparation of a silanization agent containing MCD. It is synthesized by the reaction of triethoxy(3-isocyanatopropyl)silane with MCD. Base-catalyzed silanization of superparamagnetic gamma-Fe2O3 provides a relatively stable colloid product containing 48 mu mol of MCD g(-1). MCD-modified gamma-Fe2O3 nanoparticles decrease the initial rate of the uptake and accumulation of L-[C-14]glutamate and increase the extracellular L-[C-14]glutamate level in the preparation of nerve terminals. The effect of MCD-immobilized nanoparticles is the same as that of MCD solution moreover, magnetic manipulation of the nanoparticles enables removal of bonded cholesterol.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

—

Svazek periodika

149

Číslo periodika v rámci svazku

1 January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

64-71

Kód UT WoS článku

000390625200009

EID výsledku v databázi Scopus

2-s2.0-84994385928