Curcumin-bortezomib loaded polymeric nanoparticles for synergistic cancer therapy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00475193" target="_blank" >RIV/61389013:_____/17:00475193 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/17:10361465

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.eurpolymj.2017.05.036" target="_blank" >http://dx.doi.org/10.1016/j.eurpolymj.2017.05.036</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.eurpolymj.2017.05.036" target="_blank" >10.1016/j.eurpolymj.2017.05.036</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Curcumin-bortezomib loaded polymeric nanoparticles for synergistic cancer therapy

Popis výsledku v původním jazyce

A series of well-defined methoxy-poly(ethylene glycol)-block-polylactic acid (mPEG-b-PLA) diblock copolymers were successfully synthesized, characterized and used for the construction of anticancer nanoparticle delivery system. Nanoparticles (NPs) based on these polymers were prepared by employing the nanoprecipitation method, and they were non-covalently loaded with curcumin, curcumin-bortezomib model or curcumin-bortezomib complex (curc-BTZ). Both curcumin and bortezomib are rather hydrophobic and poorly water-soluble potent anticancer drugs with synergic effects forming together a pH-sensitive complex, stable at pH of blood plasma, yet hydrolytically labile at mildly acidic milieu typical for endosomes and interstitial space in solid tumors. PEG-Curcumin-loaded and curc-BTZ-loaded NPs with 100–150 nm size showed the maximum cellular uptake by HeLa, MCF-7 and MDA-MB 231 cells after 3 h. The NPs were located in the cytoplasm of the cells but not inside the nucleus. Bare NPs did not induce any cytotoxicity in the same cell lines in in vitro experiments, even at very high concentrations (up to 800 µg/mL). NPs containing curcumin were cytotoxic with an IC50 of 25 µg/mL, which corresponds to 2.5 µg/mL of loaded curcumin. These results show that the efficacy of curcumin is significantly enhanced when using the NPs as carriers. The efficiency was further augmented through the complexation of BTZ with curcumin. When using free curc-BTZ-complex, MCF-7 cells were more sensitive to the free complex 18.8 nM (IC50) than MDA-MB-231 cells 122.4 nM (IC50). Nanoparticle formulations with these drugs caused significant cytotoxicity with 7.5 nM (IC50) and 59.2 nM (IC50) after 24 h of the treatment.

Název v anglickém jazyce

Curcumin-bortezomib loaded polymeric nanoparticles for synergistic cancer therapy

Popis výsledku anglicky

A series of well-defined methoxy-poly(ethylene glycol)-block-polylactic acid (mPEG-b-PLA) diblock copolymers were successfully synthesized, characterized and used for the construction of anticancer nanoparticle delivery system. Nanoparticles (NPs) based on these polymers were prepared by employing the nanoprecipitation method, and they were non-covalently loaded with curcumin, curcumin-bortezomib model or curcumin-bortezomib complex (curc-BTZ). Both curcumin and bortezomib are rather hydrophobic and poorly water-soluble potent anticancer drugs with synergic effects forming together a pH-sensitive complex, stable at pH of blood plasma, yet hydrolytically labile at mildly acidic milieu typical for endosomes and interstitial space in solid tumors. PEG-Curcumin-loaded and curc-BTZ-loaded NPs with 100–150 nm size showed the maximum cellular uptake by HeLa, MCF-7 and MDA-MB 231 cells after 3 h. The NPs were located in the cytoplasm of the cells but not inside the nucleus. Bare NPs did not induce any cytotoxicity in the same cell lines in in vitro experiments, even at very high concentrations (up to 800 µg/mL). NPs containing curcumin were cytotoxic with an IC50 of 25 µg/mL, which corresponds to 2.5 µg/mL of loaded curcumin. These results show that the efficacy of curcumin is significantly enhanced when using the NPs as carriers. The efficiency was further augmented through the complexation of BTZ with curcumin. When using free curc-BTZ-complex, MCF-7 cells were more sensitive to the free complex 18.8 nM (IC50) than MDA-MB-231 cells 122.4 nM (IC50). Nanoparticle formulations with these drugs caused significant cytotoxicity with 7.5 nM (IC50) and 59.2 nM (IC50) after 24 h of the treatment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Polymer Journal

ISSN

0014-3057

e-ISSN

—

Svazek periodika

93

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

116-131

Kód UT WoS článku

000407186200012

EID výsledku v databázi Scopus

2-s2.0-85019995345