PEGylation controls attachment and engulfment of monodisperse magnetic poly(2-hydroxyethyl methacrylate) microspheres by murine J774.2 macrophages

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00476589" target="_blank" >RIV/61389013:_____/17:00476589 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.apsusc.2017.07.148" target="_blank" >http://dx.doi.org/10.1016/j.apsusc.2017.07.148</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apsusc.2017.07.148" target="_blank" >10.1016/j.apsusc.2017.07.148</a>

Jazyk výsledku

angličtina

Název v původním jazyce

PEGylation controls attachment and engulfment of monodisperse magnetic poly(2-hydroxyethyl methacrylate) microspheres by murine J774.2 macrophages

Popis výsledku v původním jazyce

The first objective of this work was to prepare biocompatible magnetic polymer microspheres with reactive functional groups that could withstand nonspecific protein adsorption from biological media. Carboxyl group-containing magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) microspheres ~4 micro m in size were prepared by multistage swelling polymerization, precipitation of iron oxide inside their pores, and coating with an .alpha.-methoxy-.omega.-amino poly(ethylene glycol) (CH3O-PEG750-NH2 or CH3O-PEG5,000-NH2)/.alpha.-amino-.omega.-t-Boc-amino poly(ethylene glycol) (H2N-PEG5,000-NH-t-Boc) mixture. The mgt.PHEMA@PEG microspheres contained ~10 micro mol COOH per g. Biocompatibility of the particles was evaluated by their treatment with human embryonic kidney cells of the HEK293 line. The microspheres did not interfere with the growth of these cells, suggesting that the particles can be considered non-toxic. A second goal of this study was to address on the interaction of the developed microspheres with macrophages that commonly eliminate foreign microbodies appearing in organisms. Murine J774.2 macrophages (J774.2) were cultured in the presence of the neat and PEGylated microspheres for 2 h. Mgt.PHEMA@PEG5,000 microspheres significantly adhered to the surface of J774.2 macrophages but were minimally engulfed. Due to these properties, the mgt.PHEMA@PEG microspheres might be useful for application in drug delivery systems and monitoring of the efficiency of phagocytosis.

Název v anglickém jazyce

PEGylation controls attachment and engulfment of monodisperse magnetic poly(2-hydroxyethyl methacrylate) microspheres by murine J774.2 macrophages

Popis výsledku anglicky

The first objective of this work was to prepare biocompatible magnetic polymer microspheres with reactive functional groups that could withstand nonspecific protein adsorption from biological media. Carboxyl group-containing magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) microspheres ~4 micro m in size were prepared by multistage swelling polymerization, precipitation of iron oxide inside their pores, and coating with an .alpha.-methoxy-.omega.-amino poly(ethylene glycol) (CH3O-PEG750-NH2 or CH3O-PEG5,000-NH2)/.alpha.-amino-.omega.-t-Boc-amino poly(ethylene glycol) (H2N-PEG5,000-NH-t-Boc) mixture. The mgt.PHEMA@PEG microspheres contained ~10 micro mol COOH per g. Biocompatibility of the particles was evaluated by their treatment with human embryonic kidney cells of the HEK293 line. The microspheres did not interfere with the growth of these cells, suggesting that the particles can be considered non-toxic. A second goal of this study was to address on the interaction of the developed microspheres with macrophages that commonly eliminate foreign microbodies appearing in organisms. Murine J774.2 macrophages (J774.2) were cultured in the presence of the neat and PEGylated microspheres for 2 h. Mgt.PHEMA@PEG5,000 microspheres significantly adhered to the surface of J774.2 macrophages but were minimally engulfed. Due to these properties, the mgt.PHEMA@PEG microspheres might be useful for application in drug delivery systems and monitoring of the efficiency of phagocytosis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Surface Science

ISSN

0169-4332

e-ISSN

—

Svazek periodika

426

Číslo periodika v rámci svazku

31 December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

315-324

Kód UT WoS článku

000413658200035

EID výsledku v databázi Scopus

2-s2.0-85026406051