Biological evaluation of surface-modified magnetic nanoparticles as a platform for colon cancer cell theranostics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00482869" target="_blank" >RIV/61389013:_____/18:00482869 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.colsurfb.2017.10.034" target="_blank" >http://dx.doi.org/10.1016/j.colsurfb.2017.10.034</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2017.10.034" target="_blank" >10.1016/j.colsurfb.2017.10.034</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biological evaluation of surface-modified magnetic nanoparticles as a platform for colon cancer cell theranostics

Popis výsledku v původním jazyce

Magnetic nanoparticles offer multiple possibilities for biomedical applications. Besides their physico-chemical properties, nanoparticle-cellular interactions are determinant for biological safety. In this work, magnetic nanoparticles were synthesized by one-shot precipitation or two-step reaction and coated with biocompatible polymers, such as poly(l-lysine) and poly(N,N-dimethylacrylamide-co-acrylic acid), and carbohydrates, like l-ascorbic acid, d-galactose, d-mannose, and sucrose. The resulting magnetic nanoparticles were characterized by dynamic light scattering, FT-Raman spectroscopy, transmission electron microscopy, SQUID magnetometry, and Mössbauer spectroscopy. Ability of the nanoparticles to be used in theranostic applications was also evaluated, showing that coating with biocompatible polymers increased the heating efficiency. Nanoparticles synthesized by one-shot precipitation were 50% larger (∼13 nm) than those obtained by a two-step reaction (∼8 nm). Magnetic nanoparticles at concentrations up to 500 μg mL−1 were non-cytotoxic to L929 fibroblasts. Particles synthesized by one-shot precipitation had little effect on viability, cell cycle and apoptosis of the three human colon cancer cell lines used: Caco-2, HT-29, and SW-480. At the same concentration (500 μg mL−1), magnetic particles prepared by a two-step reaction reduced colon cancer cell viability by 20%, affecting cell cycle and inducing cell apoptosis. Uptake of surface-coated magnetic nanoparticles by colon cancer cells was dependent on particle synthesis, surface coating and incubation time.

Název v anglickém jazyce

Biological evaluation of surface-modified magnetic nanoparticles as a platform for colon cancer cell theranostics

Popis výsledku anglicky

Magnetic nanoparticles offer multiple possibilities for biomedical applications. Besides their physico-chemical properties, nanoparticle-cellular interactions are determinant for biological safety. In this work, magnetic nanoparticles were synthesized by one-shot precipitation or two-step reaction and coated with biocompatible polymers, such as poly(l-lysine) and poly(N,N-dimethylacrylamide-co-acrylic acid), and carbohydrates, like l-ascorbic acid, d-galactose, d-mannose, and sucrose. The resulting magnetic nanoparticles were characterized by dynamic light scattering, FT-Raman spectroscopy, transmission electron microscopy, SQUID magnetometry, and Mössbauer spectroscopy. Ability of the nanoparticles to be used in theranostic applications was also evaluated, showing that coating with biocompatible polymers increased the heating efficiency. Nanoparticles synthesized by one-shot precipitation were 50% larger (∼13 nm) than those obtained by a two-step reaction (∼8 nm). Magnetic nanoparticles at concentrations up to 500 μg mL−1 were non-cytotoxic to L929 fibroblasts. Particles synthesized by one-shot precipitation had little effect on viability, cell cycle and apoptosis of the three human colon cancer cell lines used: Caco-2, HT-29, and SW-480. At the same concentration (500 μg mL−1), magnetic particles prepared by a two-step reaction reduced colon cancer cell viability by 20%, affecting cell cycle and inducing cell apoptosis. Uptake of surface-coated magnetic nanoparticles by colon cancer cells was dependent on particle synthesis, surface coating and incubation time.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GC16-01128J" target="_blank" >GC16-01128J: Magnetické nanočástice s antioxidačním účinkem na bázi přírodních antioxidantů: Interakce nanočástic s buňkami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

—

Svazek periodika

161

Číslo periodika v rámci svazku

1 January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

35-41

Kód UT WoS článku

000423636100005

EID výsledku v databázi Scopus

2-s2.0-85032858139