Vše

Co hledáte?

Vše
Projekty
Výsledky výzkumu
Subjekty

Rychlé hledání

  • Projekty podpořené TA ČR
  • Významné projekty
  • Projekty s nejvyšší státní podporou
  • Aktuálně běžící projekty

Chytré vyhledávání

  • Takto najdu konkrétní +slovo
  • Takto z výsledků -slovo zcela vynechám
  • “Takto můžu najít celou frázi”

Biocompatibility assessment of up-and down-converting nanoparticles: implications of interferences with in vitro assays

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00497918" target="_blank" >RIV/61389013:_____/19:00497918 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://iopscience.iop.org/article/10.1088/2050-6120/aae9c8" target="_blank" >https://iopscience.iop.org/article/10.1088/2050-6120/aae9c8</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1088/2050-6120/aae9c8" target="_blank" >10.1088/2050-6120/aae9c8</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Biocompatibility assessment of up-and down-converting nanoparticles: implications of interferences with in vitro assays

  • Popis výsledku v původním jazyce

    The safety assessment of nanoparticles (NPs) is crucial during their design and development for biomedicine. One of the prerequisite steps during this evaluation is in vitro testing that employs cell-based assays not always validated and well-adapted for NPs. Interferences with in vitro assays may arise due to the nano-related optical, oxidative, fluorescent, surface and catalytic properties of NPs. Thus, proper validation of each assay system has to be performed for each NP type. This study aimed to evaluate the applicability of the most common in vitro cytotoxicity assays for the safety assessment of up- and down-converting lanthanide-doped NPs. Conventional cell viability tests and fluorescence-based assays for oxidative stress response were selected to determine the biological effects of up- and down-converting NPs to human brain cells. Comparison with known silver and iron oxide NPs was made for verification purposes. Both the plate reader and flow cytometric measurements were examined. The obtained results indicated that both types of Ln-doped NPs interfered to a much lesser extent than metallic NPs. In addition, the great potential of both up- and down-converting NPs for biomedicine was manifested due to their biocompatibility and low toxicity.

  • Název v anglickém jazyce

    Biocompatibility assessment of up-and down-converting nanoparticles: implications of interferences with in vitro assays

  • Popis výsledku anglicky

    The safety assessment of nanoparticles (NPs) is crucial during their design and development for biomedicine. One of the prerequisite steps during this evaluation is in vitro testing that employs cell-based assays not always validated and well-adapted for NPs. Interferences with in vitro assays may arise due to the nano-related optical, oxidative, fluorescent, surface and catalytic properties of NPs. Thus, proper validation of each assay system has to be performed for each NP type. This study aimed to evaluate the applicability of the most common in vitro cytotoxicity assays for the safety assessment of up- and down-converting lanthanide-doped NPs. Conventional cell viability tests and fluorescence-based assays for oxidative stress response were selected to determine the biological effects of up- and down-converting NPs to human brain cells. Comparison with known silver and iron oxide NPs was made for verification purposes. Both the plate reader and flow cytometric measurements were examined. The obtained results indicated that both types of Ln-doped NPs interfered to a much lesser extent than metallic NPs. In addition, the great potential of both up- and down-converting NPs for biomedicine was manifested due to their biocompatibility and low toxicity.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10404 - Polymer science

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2019

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Methods and Applications in Fluorescence

  • ISSN

    2050-6120

  • e-ISSN

  • Svazek periodika

    7

  • Číslo periodika v rámci svazku

    1

  • Stát vydavatele periodika

    GB - Spojené království Velké Británie a Severního Irska

  • Počet stran výsledku

    15

  • Strana od-do

    1-15

  • Kód UT WoS článku

    000517123400001

  • EID výsledku v databázi Scopus

    2-s2.0-85056273391