Biocompatibility assessment of up-and down-converting nanoparticles: implications of interferences with in vitro assays
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00497918" target="_blank" >RIV/61389013:_____/19:00497918 - isvavai.cz</a>
Výsledek na webu
<a href="https://iopscience.iop.org/article/10.1088/2050-6120/aae9c8" target="_blank" >https://iopscience.iop.org/article/10.1088/2050-6120/aae9c8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/2050-6120/aae9c8" target="_blank" >10.1088/2050-6120/aae9c8</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Biocompatibility assessment of up-and down-converting nanoparticles: implications of interferences with in vitro assays
Popis výsledku v původním jazyce
The safety assessment of nanoparticles (NPs) is crucial during their design and development for biomedicine. One of the prerequisite steps during this evaluation is in vitro testing that employs cell-based assays not always validated and well-adapted for NPs. Interferences with in vitro assays may arise due to the nano-related optical, oxidative, fluorescent, surface and catalytic properties of NPs. Thus, proper validation of each assay system has to be performed for each NP type. This study aimed to evaluate the applicability of the most common in vitro cytotoxicity assays for the safety assessment of up- and down-converting lanthanide-doped NPs. Conventional cell viability tests and fluorescence-based assays for oxidative stress response were selected to determine the biological effects of up- and down-converting NPs to human brain cells. Comparison with known silver and iron oxide NPs was made for verification purposes. Both the plate reader and flow cytometric measurements were examined. The obtained results indicated that both types of Ln-doped NPs interfered to a much lesser extent than metallic NPs. In addition, the great potential of both up- and down-converting NPs for biomedicine was manifested due to their biocompatibility and low toxicity.
Název v anglickém jazyce
Biocompatibility assessment of up-and down-converting nanoparticles: implications of interferences with in vitro assays
Popis výsledku anglicky
The safety assessment of nanoparticles (NPs) is crucial during their design and development for biomedicine. One of the prerequisite steps during this evaluation is in vitro testing that employs cell-based assays not always validated and well-adapted for NPs. Interferences with in vitro assays may arise due to the nano-related optical, oxidative, fluorescent, surface and catalytic properties of NPs. Thus, proper validation of each assay system has to be performed for each NP type. This study aimed to evaluate the applicability of the most common in vitro cytotoxicity assays for the safety assessment of up- and down-converting lanthanide-doped NPs. Conventional cell viability tests and fluorescence-based assays for oxidative stress response were selected to determine the biological effects of up- and down-converting NPs to human brain cells. Comparison with known silver and iron oxide NPs was made for verification purposes. Both the plate reader and flow cytometric measurements were examined. The obtained results indicated that both types of Ln-doped NPs interfered to a much lesser extent than metallic NPs. In addition, the great potential of both up- and down-converting NPs for biomedicine was manifested due to their biocompatibility and low toxicity.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10404 - Polymer science
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Methods and Applications in Fluorescence
ISSN
2050-6120
e-ISSN
—
Svazek periodika
7
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
15
Strana od-do
1-15
Kód UT WoS článku
000517123400001
EID výsledku v databázi Scopus
2-s2.0-85056273391