pH-Dependent disruption of giant polymer vesicles: a step towards biomimetic membranes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00567447" target="_blank" >RIV/61389013:_____/23:00567447 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlelanding/2023/PY/D2PY01229D" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2023/PY/D2PY01229D</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/D2PY01229D" target="_blank" >10.1039/D2PY01229D</a>

Jazyk výsledku

angličtina

Název v původním jazyce

pH-Dependent disruption of giant polymer vesicles: a step towards biomimetic membranes

Popis výsledku v původním jazyce

Giant unilamellar polymer vesicles (GUPVs) capable of selectively delivering protected payloads into intracellular environments and releasing them in stimuli-triggered, precise spatially and temporally controlled manners are attractive bioactive cargo delivery tools. Herein, we present highly size-defined and monodisperse (42.1 ± 1.2, 60.5 ± 1.0, 80.4 ± 1.4 and 97.9 ± 1.2 μm in diameter, respectively) pH-responsive giant GUPVs prepared via microfluidic droplet generation using a flow-focusing poly(dimethylsiloxane) (PDMS)-based microfluidic device. Poly(ethylene oxide)-block-poly[2-(diisopropylamino)ethyl methacrylate] (PEO-b-PDPA) is a pH-responsive polymer that was synthesized via reversible addition–fragmentation chain-transfer (RAFT) polymerization and used in combination with poly(ethylene oxide)-block-poly(1,2-butadiene) (PEO-b-PBD) to produce homogeneous pH-responsive giant GUPVs. To demonstrate the spatiotemporal control provided by this approach, we studied in detail the pH-responsiveness of GUPVs according to the disruption and release of dye cargo under distinct acidic conditions using fluorescence confocal microscopy. This approach can be utilized to fabricate pH-responsive delivery systems for various active compounds, microreactors, and artificial organelles, thereby paving the way towards cell mimicry.n

Název v anglickém jazyce

pH-Dependent disruption of giant polymer vesicles: a step towards biomimetic membranes

Popis výsledku anglicky

Giant unilamellar polymer vesicles (GUPVs) capable of selectively delivering protected payloads into intracellular environments and releasing them in stimuli-triggered, precise spatially and temporally controlled manners are attractive bioactive cargo delivery tools. Herein, we present highly size-defined and monodisperse (42.1 ± 1.2, 60.5 ± 1.0, 80.4 ± 1.4 and 97.9 ± 1.2 μm in diameter, respectively) pH-responsive giant GUPVs prepared via microfluidic droplet generation using a flow-focusing poly(dimethylsiloxane) (PDMS)-based microfluidic device. Poly(ethylene oxide)-block-poly[2-(diisopropylamino)ethyl methacrylate] (PEO-b-PDPA) is a pH-responsive polymer that was synthesized via reversible addition–fragmentation chain-transfer (RAFT) polymerization and used in combination with poly(ethylene oxide)-block-poly(1,2-butadiene) (PEO-b-PBD) to produce homogeneous pH-responsive giant GUPVs. To demonstrate the spatiotemporal control provided by this approach, we studied in detail the pH-responsiveness of GUPVs according to the disruption and release of dye cargo under distinct acidic conditions using fluorescence confocal microscopy. This approach can be utilized to fabricate pH-responsive delivery systems for various active compounds, microreactors, and artificial organelles, thereby paving the way towards cell mimicry.n

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Polymer Chemistry

ISSN

1759-9954

e-ISSN

1759-9962

Svazek periodika

14

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

443-451

Kód UT WoS článku

000899359800001

EID výsledku v databázi Scopus

2-s2.0-85144786973