Cryopreservation of apheresis platelets treated with riboflavin and UV light

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00570648" target="_blank" >RIV/61389013:_____/23:00570648 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/24:00558342 RIV/61383082:_____/23:00001274 RIV/68407700:21460/23:00373168 RIV/00216208:11110/23:10465966 a 2 dalších

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1473050222002798?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1473050222002798?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.transci.2022.103580" target="_blank" >10.1016/j.transci.2022.103580</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of apheresis platelets treated with riboflavin and UV light

Popis výsledku v původním jazyce

Pathogen reduction technology (PRT) is increasingly used in the preparation of platelets for therapeutic transfusion. As the Czech Republic considers PRT, we asked what effects PRT may have on the recovery and function of platelets after cryopreservation (CP), which we use in both military and civilian blood settings. 16 Group O apheresis platelets units were treated with PRT (Mirasol, Terumo BCT, USA) before freezing, 15 similarly collected units were frozen without PRT as controls. All units were processed with 5–6% DMSO, frozen at − 80 °C, stored > 14 days, and reconstituted in thawed AB plasma. After reconstitution, all units were assessed for: platelet count, mean platelet volume (MPV), platelet recovery, thromboelastography, thrombin generation time, endogenous thrombin potential (ETP), glucose, lactate, pH, pO2, pCO2, HCO3, CD41, CD42b, CD62, Annexin V, CCL5, CD62P, and aggregates > 2 mm and selected units for Kunicki score. PRT treated platelet units had lower platelet number (247 vs 278 ×109/U), reduced thromboelastographic MA (38 vs 62 mm) and demonstrated aggregates compared to untreated platelets. Plasma coagulation functions were largely unchanged. Samples from PRT units showed reduced platelet number, reduced function greater than the reduced number would cause, and aggregates. While the platelet numbers are sufficient to meet the European standard, marked platelets activation with weak clot strength suggest reduced effectiveness.

Název v anglickém jazyce

Cryopreservation of apheresis platelets treated with riboflavin and UV light

Popis výsledku anglicky

Pathogen reduction technology (PRT) is increasingly used in the preparation of platelets for therapeutic transfusion. As the Czech Republic considers PRT, we asked what effects PRT may have on the recovery and function of platelets after cryopreservation (CP), which we use in both military and civilian blood settings. 16 Group O apheresis platelets units were treated with PRT (Mirasol, Terumo BCT, USA) before freezing, 15 similarly collected units were frozen without PRT as controls. All units were processed with 5–6% DMSO, frozen at − 80 °C, stored > 14 days, and reconstituted in thawed AB plasma. After reconstitution, all units were assessed for: platelet count, mean platelet volume (MPV), platelet recovery, thromboelastography, thrombin generation time, endogenous thrombin potential (ETP), glucose, lactate, pH, pO2, pCO2, HCO3, CD41, CD42b, CD62, Annexin V, CCL5, CD62P, and aggregates > 2 mm and selected units for Kunicki score. PRT treated platelet units had lower platelet number (247 vs 278 ×109/U), reduced thromboelastographic MA (38 vs 62 mm) and demonstrated aggregates compared to untreated platelets. Plasma coagulation functions were largely unchanged. Samples from PRT units showed reduced platelet number, reduced function greater than the reduced number would cause, and aggregates. While the platelet numbers are sufficient to meet the European standard, marked platelets activation with weak clot strength suggest reduced effectiveness.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Transfusion and Apheresis Science

ISSN

1473-0502

e-ISSN

1878-1683

Svazek periodika

62

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

103580

Kód UT WoS článku

000996453300001

EID výsledku v databázi Scopus

2-s2.0-85138766172