Pathogen reduction of Frozen Platelets

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F19%3A00338742" target="_blank" >RIV/68407700:21460/19:00338742 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/journal/14230410" target="_blank" >https://onlinelibrary.wiley.com/journal/14230410</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Pathogen reduction of Frozen Platelets

Popis výsledku v původním jazyce

Aims: This study explores the impact of Pathogen reduction technology (PRT) on subsequent freezing of platelets. The goal was a comparison of in vitro quality parameters between PRT treated and untreated platelets. Methods: A comparative study of CP, PRT treated (T-CP) and untreated (C-CP), was performed. Both arms of the study were collected as apheresis PLTs type O on a Haemonetics MCS+ machine. Type-O CPs were processed with 6% DMSO, frozen at -80°C and reconstituted in thawed AB plasma. 16 units were treated with PRT (Mirasol, Terumo BCT, USA) before freezing, and 15 CPs in the control group were left untreated. After reconstitution of CPs the following laboratory tests were performed: blood cells count, PLT, MPV, PLT recovery, glucose, lactate, pH, pO2, pCO2, HCO3, TEG, TGT, CD41, CD42b, Annexin V, CCL5, CD62P, Kunicky score and presence of aggregates > 2 mm. Results: No significant differences between both groups were found for the following parameters: number of PLT / unit, recovery, MPV, pH, TGT – Peak, CD41 and Annexin V. T-CPs showed significantly higher TEG coagulation index, TGT – tLag with faster tPeak, velocity index and ETP as well as metabolic markers were increased, evidenced by lower pO2, pCO2, glucose, lactate and higher HCO3. Expression of CCL5 and sCD62P were elevated in T-CPs when compared to C-CPs. Morphological changes evaluated by Kunicky score was higher in T-CPs. All units of T-CP contained visible aggregates, while no aggregates were seen in C-CP. Conclusion: T-CPs showed better in vitro hemostatic activity with less morphological changes than their untreated counterparts. T-CPs seem to be more metabolic activated than C-CPs, however platelet functionality did not seem impaired. On the other hand, the occurrence of aggregates in T-CPs probably needs further research or adaptation of preparation procedures. In the meantime T-CTs should be administered early after thawing with an active hemovigilance in place.

Název v anglickém jazyce

Pathogen reduction of Frozen Platelets

Popis výsledku anglicky

Aims: This study explores the impact of Pathogen reduction technology (PRT) on subsequent freezing of platelets. The goal was a comparison of in vitro quality parameters between PRT treated and untreated platelets. Methods: A comparative study of CP, PRT treated (T-CP) and untreated (C-CP), was performed. Both arms of the study were collected as apheresis PLTs type O on a Haemonetics MCS+ machine. Type-O CPs were processed with 6% DMSO, frozen at -80°C and reconstituted in thawed AB plasma. 16 units were treated with PRT (Mirasol, Terumo BCT, USA) before freezing, and 15 CPs in the control group were left untreated. After reconstitution of CPs the following laboratory tests were performed: blood cells count, PLT, MPV, PLT recovery, glucose, lactate, pH, pO2, pCO2, HCO3, TEG, TGT, CD41, CD42b, Annexin V, CCL5, CD62P, Kunicky score and presence of aggregates > 2 mm. Results: No significant differences between both groups were found for the following parameters: number of PLT / unit, recovery, MPV, pH, TGT – Peak, CD41 and Annexin V. T-CPs showed significantly higher TEG coagulation index, TGT – tLag with faster tPeak, velocity index and ETP as well as metabolic markers were increased, evidenced by lower pO2, pCO2, glucose, lactate and higher HCO3. Expression of CCL5 and sCD62P were elevated in T-CPs when compared to C-CPs. Morphological changes evaluated by Kunicky score was higher in T-CPs. All units of T-CP contained visible aggregates, while no aggregates were seen in C-CP. Conclusion: T-CPs showed better in vitro hemostatic activity with less morphological changes than their untreated counterparts. T-CPs seem to be more metabolic activated than C-CPs, however platelet functionality did not seem impaired. On the other hand, the occurrence of aggregates in T-CPs probably needs further research or adaptation of preparation procedures. In the meantime T-CTs should be administered early after thawing with an active hemovigilance in place.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30205 - Hematology

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů