HPMA-based nitroxide radical containing nanoparticles with controlled radical release: detailed physico-chemical characterization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F25%3A00604458" target="_blank" >RIV/61389013:_____/25:00604458 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/25:10492822

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0014305725000151?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014305725000151?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.eurpolymj.2025.113727" target="_blank" >10.1016/j.eurpolymj.2025.113727</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HPMA-based nitroxide radical containing nanoparticles with controlled radical release: detailed physico-chemical characterization

Popis výsledku v původním jazyce

This research focuses on the measurement of the release of nitroxide radicals from polymer-based nanoparticles by electron paramagnetic resonance (EPR) and potentiometric titration techniques. Nitroxide radicals such as 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) are known to modulate oxidative stress by scavenging reactive oxygen species that cause cell damage. It was also found that these biologically active radicals can inhibit tumor growth by inducing apoptosis in cancer cells. Nitroxide radicals, however, cannot be effectively used due to their non-specific accumulation in normal tissue and quick elimination from the body. To address these challenges, we developed pH-responsive nanoparticles based on self-assembly of amphiphilic N-(2-hydroxypropyl)methacrylamide (HPMA)-based copolymers containing TEMPO radicals, covalently attached to the hydrophilic shell by a pH-sensitive hydrazone bond, and compared with pH-unresponsive nanoparticles, where the TEMPO radicals were bound by a physiologically stable amide bond. We thoroughly investigated the nitroxide radicals release from nanoparticles by EPR and potentiometric titration for the first time. TEMPO radicals were almost completely released from the pH-responsive nanoparticles within three hours at pH = 5 at 37 °C, which corresponds to tumor tissue, while only quite slow release of radicals was observed at physiological pH within 24 h. The results show that nitroxide radicals containing HPMA-based nanoparticles could be potentially used either for tumor visualization or as smart prodrugs to enhance nitroxide radicals’ therapeutic efficacy and safety.

Název v anglickém jazyce

HPMA-based nitroxide radical containing nanoparticles with controlled radical release: detailed physico-chemical characterization

Popis výsledku anglicky

This research focuses on the measurement of the release of nitroxide radicals from polymer-based nanoparticles by electron paramagnetic resonance (EPR) and potentiometric titration techniques. Nitroxide radicals such as 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) are known to modulate oxidative stress by scavenging reactive oxygen species that cause cell damage. It was also found that these biologically active radicals can inhibit tumor growth by inducing apoptosis in cancer cells. Nitroxide radicals, however, cannot be effectively used due to their non-specific accumulation in normal tissue and quick elimination from the body. To address these challenges, we developed pH-responsive nanoparticles based on self-assembly of amphiphilic N-(2-hydroxypropyl)methacrylamide (HPMA)-based copolymers containing TEMPO radicals, covalently attached to the hydrophilic shell by a pH-sensitive hydrazone bond, and compared with pH-unresponsive nanoparticles, where the TEMPO radicals were bound by a physiologically stable amide bond. We thoroughly investigated the nitroxide radicals release from nanoparticles by EPR and potentiometric titration for the first time. TEMPO radicals were almost completely released from the pH-responsive nanoparticles within three hours at pH = 5 at 37 °C, which corresponds to tumor tissue, while only quite slow release of radicals was observed at physiological pH within 24 h. The results show that nitroxide radicals containing HPMA-based nanoparticles could be potentially used either for tumor visualization or as smart prodrugs to enhance nitroxide radicals’ therapeutic efficacy and safety.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Polymer Journal

ISSN

0014-3057

e-ISSN

1873-1945

Svazek periodika

225

Číslo periodika v rámci svazku

14 February

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

113727

Kód UT WoS článku

001411394500001

EID výsledku v databázi Scopus

2-s2.0-85214333024