Dual thermo- and ROS-responsive triblock copolymers as 19F MRI tracers for functional nanoparticles and hydrogels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F25%3A00638465" target="_blank" >RIV/61389013:_____/25:00638465 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/25:00638465 RIV/00216208:11110/25:10501933

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0014305725004999?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014305725004999?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.eurpolymj.2025.114211" target="_blank" >10.1016/j.eurpolymj.2025.114211</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dual thermo- and ROS-responsive triblock copolymers as 19F MRI tracers for functional nanoparticles and hydrogels

Popis výsledku v původním jazyce

Theranostic systems that integrate therapeutic delivery with diagnostic imaging hold strong potential in biomedical applications. Nanocarriers with imaging and controlled release functions enable real-time tracking and localization of therapeutics, while hydrogels with diagnostic capabilities support applications such as sustained drug release, cell encapsulation, and cell tracking. Fluorine-19 magnetic resonance imaging (19F-MRI) is a promising non-invasive complement to conventional proton MRI, though its clinical translation remains limited by the lack of optimal tracer systems. Herein, we report the development of BAB-type triblock copolymers comprising a hydrophilic poly(2-ethyl-2-oxazoline) (PEtOx) A block and a thermoresponsive poly[N-(2,2-difluoroethyl)acrylamide] (PDFEA) B block, statistically copolymerized with a ROS-responsive monomer bearing phenylboronic ester groups. These polymers self-assemble into nanoparticles at low concentrations and form thermogelling hydrogels at higher concentrations, allowing for formulation-dependent versatility. The ROS-sensitive component enables disassembly at pathophysiologically relevant ROS levels (∼0.4–2 mM), facilitating targeted therapeutic release in oxidative environments such as tumors. The polymers form physically crosslinked nanogels (hydrodynamic radius ≈160–760 nm) at 37 °C, which undergo ROS-triggered disassembly. Selected formulations demonstrated excellent 19F-MRI relaxation properties suitable for in vivo imaging. Cytocompatibility was confirmed in vitro using human foreskin fibroblasts. Overall, the developed polymers offer a versatile platform for biomedical applications—ranging from thermogelling injectable hydrogels for drug delivery or cell encapsulation, to nanocarriers for ROS-triggered therapeutic release—all while enabling non-invasive monitoring via 19F-MRI.

Název v anglickém jazyce

Dual thermo- and ROS-responsive triblock copolymers as 19F MRI tracers for functional nanoparticles and hydrogels

Popis výsledku anglicky

Theranostic systems that integrate therapeutic delivery with diagnostic imaging hold strong potential in biomedical applications. Nanocarriers with imaging and controlled release functions enable real-time tracking and localization of therapeutics, while hydrogels with diagnostic capabilities support applications such as sustained drug release, cell encapsulation, and cell tracking. Fluorine-19 magnetic resonance imaging (19F-MRI) is a promising non-invasive complement to conventional proton MRI, though its clinical translation remains limited by the lack of optimal tracer systems. Herein, we report the development of BAB-type triblock copolymers comprising a hydrophilic poly(2-ethyl-2-oxazoline) (PEtOx) A block and a thermoresponsive poly[N-(2,2-difluoroethyl)acrylamide] (PDFEA) B block, statistically copolymerized with a ROS-responsive monomer bearing phenylboronic ester groups. These polymers self-assemble into nanoparticles at low concentrations and form thermogelling hydrogels at higher concentrations, allowing for formulation-dependent versatility. The ROS-sensitive component enables disassembly at pathophysiologically relevant ROS levels (∼0.4–2 mM), facilitating targeted therapeutic release in oxidative environments such as tumors. The polymers form physically crosslinked nanogels (hydrodynamic radius ≈160–760 nm) at 37 °C, which undergo ROS-triggered disassembly. Selected formulations demonstrated excellent 19F-MRI relaxation properties suitable for in vivo imaging. Cytocompatibility was confirmed in vitro using human foreskin fibroblasts. Overall, the developed polymers offer a versatile platform for biomedical applications—ranging from thermogelling injectable hydrogels for drug delivery or cell encapsulation, to nanocarriers for ROS-triggered therapeutic release—all while enabling non-invasive monitoring via 19F-MRI.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Polymer Journal

ISSN

0014-3057

e-ISSN

1873-1945

Svazek periodika

238

Číslo periodika v rámci svazku

24 September

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

114211

Kód UT WoS článku

001688206000001

EID výsledku v databázi Scopus

2-s2.0-105013252123