Chloramines of amino acids as precursors of bactericidal activity in cell culture media DMEM and RPMI treated by RF-driven He/O<inf>2</inf> plasma jet

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389021%3A_____%2F23%3A00573458" target="_blank" >RIV/61389021:_____/23:00573458 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/ppap.202300052" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/ppap.202300052</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ppap.202300052" target="_blank" >10.1002/ppap.202300052</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chloramines of amino acids as precursors of bactericidal activity in cell culture media DMEM and RPMI treated by RF-driven He/O<inf>2</inf> plasma jet

Popis výsledku v původním jazyce

Antibacterial properties and chemical changes of two culture media, Dulbecco's Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI), treated by RF-driven He/O2 plasma jet, were studied using various chemical diagnostics and assessing Escherichia coli inactivation. The antibacterial effect increased with the plasma treatment time and was stronger in RPMI than in DMEM. Formation of organic mono- and dichloramines of amino acids in DMEM/RPMI by hypochlorite produced by the reaction of plasma-generated O atoms with Cl− ions present in culture media were the major causes of the biocidal activity. This behavior was attributed to the action of tertiary chemical products formed by the decay of organic dichloramines. The thiobarbituric acid assay revealed malondialdehyde formation from glucose oxidation as the major component of the media.

Název v anglickém jazyce

Chloramines of amino acids as precursors of bactericidal activity in cell culture media DMEM and RPMI treated by RF-driven He/O<inf>2</inf> plasma jet

Popis výsledku anglicky

Antibacterial properties and chemical changes of two culture media, Dulbecco's Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI), treated by RF-driven He/O2 plasma jet, were studied using various chemical diagnostics and assessing Escherichia coli inactivation. The antibacterial effect increased with the plasma treatment time and was stronger in RPMI than in DMEM. Formation of organic mono- and dichloramines of amino acids in DMEM/RPMI by hypochlorite produced by the reaction of plasma-generated O atoms with Cl− ions present in culture media were the major causes of the biocidal activity. This behavior was attributed to the action of tertiary chemical products formed by the decay of organic dichloramines. The thiobarbituric acid assay revealed malondialdehyde formation from glucose oxidation as the major component of the media.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GA19-25026S" target="_blank" >GA19-25026S: Charakterizace chemicky reaktivních kapalin produkovaných nerovnovážným plazmatem</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plasma Processes and Polymers

ISSN

1612-8850

e-ISSN

1612-8869

Svazek periodika

20

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

e2300052

Kód UT WoS článku

001003514200001

EID výsledku v databázi Scopus

2-s2.0-85163111051