The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033082" target="_blank" >RIV/61389030:_____/03:56033082 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene.

Popis výsledku v původním jazyce

A simple and reliable procedure for reverse transcription-polymerase chain reaction (RT-PCR) detection and strain differentiation of Potato virus Y (PVY) was developed. Three primers were designed within the coat protein (CP) and nuclear inclusion protein b (NIb) region, exploiting a single base polymorphism identified as being present in all the recombinant PVYNTN isolates published. Samples infected with PVY produce a single band of 569 bp, while isolates belonging to PVYNTN strain give an additionalband of 334 bp. The technique was tested on a collection of well-characterised isolates of PVY from a range of strains and was found to detect all of the isolates reported as belonging to the PVYNTN strain. All of the isolates detected possess a recombination event within the coat protein. Further sequence analysis revealed that all the recombinant PVYNTN isolates reported thus far would be detected using this assay, whilst isolates thought to be PVYNTN that do not possess the coat prote

Název v anglickém jazyce

The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene.

Popis výsledku anglicky

A simple and reliable procedure for reverse transcription-polymerase chain reaction (RT-PCR) detection and strain differentiation of Potato virus Y (PVY) was developed. Three primers were designed within the coat protein (CP) and nuclear inclusion protein b (NIb) region, exploiting a single base polymorphism identified as being present in all the recombinant PVYNTN isolates published. Samples infected with PVY produce a single band of 569 bp, while isolates belonging to PVYNTN strain give an additionalband of 334 bp. The technique was tested on a collection of well-characterised isolates of PVY from a range of strains and was found to detect all of the isolates reported as belonging to the PVYNTN strain. All of the isolates detected possess a recombination event within the coat protein. Further sequence analysis revealed that all the recombinant PVYNTN isolates reported thus far would be detected using this assay, whilst isolates thought to be PVYNTN that do not possess the coat prote

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Virological Methods

ISSN

0166-0934

e-ISSN

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Svazek periodika

109

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

63-68

Kód UT WoS článku

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EID výsledku v databázi Scopus

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