Detekce nestability ploidie embryogenních buněk banánovníku pomocí průtokové cytometrie

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00107484" target="_blank" >RIV/61389030:_____/04:00107484 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Detecting ploidy level instability of banana embryogenic cell suspension cultures by flow cytometry

Popis výsledku v původním jazyce

During micropropagation of bananas and plantains, somaclonal variation can occur in regenerated plantlets. This variation may interfere with the use of these cultures for physical or chemical mutagenesis and/or genetic transformation. Although the causesof genetic instability are poorly understood, chromosome instability is believed to be one of the most common causes of tissue culture-induced variation. Using flow cytometry, variation in chromosome number could be detected in embryogenic cell suspensions and in plants regenerated from them. Results obtained by flow cytometry were verified by chromosome counting in meristem root-tip cells. Abnormalities in DNA content such as polyploidy and aneuploidy were detected at the cell suspension level. For the first time, a hypotriploid banana embryogenic cell line with 2n = 28 (i.e. with loss of five chromosomes) was reported. Factors affecting the genetic stability of embryogenic cell suspensions are discussed.

Název v anglickém jazyce

Detecting ploidy level instability of banana embryogenic cell suspension cultures by flow cytometry

Popis výsledku anglicky

During micropropagation of bananas and plantains, somaclonal variation can occur in regenerated plantlets. This variation may interfere with the use of these cultures for physical or chemical mutagenesis and/or genetic transformation. Although the causesof genetic instability are poorly understood, chromosome instability is believed to be one of the most common causes of tissue culture-induced variation. Using flow cytometry, variation in chromosome number could be detected in embryogenic cell suspensions and in plants regenerated from them. Results obtained by flow cytometry were verified by chromosome counting in meristem root-tip cells. Abnormalities in DNA content such as polyploidy and aneuploidy were detected at the cell suspension level. For the first time, a hypotriploid banana embryogenic cell line with 2n = 28 (i.e. with loss of five chromosomes) was reported. Factors affecting the genetic stability of embryogenic cell suspensions are discussed.

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/IAA6038204" target="_blank" >IAA6038204: Konstrukce knihovny DNA banánovníku klonované ve vektoru BAC (Bacterial artificial chromosome) pro fyzické mapování genomu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Banana Improvement: Cellular, Molecular Biology, and Induced Mutations

ISBN

1-57808-340-0

Počet stran výsledku

11

Strana od-do

251-261

Počet stran knihy

—

Název nakladatele

Science Publishers, Inc

Místo vydání

Enfield

Kód UT WoS kapitoly

—