Použití suspenzí buněčných kultur pro indukci a selekci mutantů u rodu Musa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00107481" target="_blank" >RIV/61389030:_____/04:00107481 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Usefulness of embryogenic cell suspension cultures for the induction and selection of mutations in Musa spp

Popis výsledku v původním jazyce

In bananas shoot-tip cultures are traditionally used for mutagenesis. The main problem with this type of culture is the presence of chimerism. In contrast, embryogenic cell suspensions allow the handling of large populations under controlled conditions and simultaneously avoid chimerism if embryos have a single-cell origin. In this study, we verified the unicellular origin of somatic embryos by treating embryogenic cell suspensions (ECS) with colchicine and determining the ploidy of regenerated plants by flow cytometry. We have found that none of the plants regenerated from colchicine-treated ECS was mixoploid (chimeric). To optimise a protocol for the irradiation of ECS, several experiments were performed. Flow cytometric analysis of cell-cycle kinetics proved to be more informative than growth curves for the determination of the optimal timing of irradiation. The optimal irradiation dose was determined using three different parameters. Among them, the fresh weight gain and the reg...

Název v anglickém jazyce

Usefulness of embryogenic cell suspension cultures for the induction and selection of mutations in Musa spp

Popis výsledku anglicky

In bananas shoot-tip cultures are traditionally used for mutagenesis. The main problem with this type of culture is the presence of chimerism. In contrast, embryogenic cell suspensions allow the handling of large populations under controlled conditions and simultaneously avoid chimerism if embryos have a single-cell origin. In this study, we verified the unicellular origin of somatic embryos by treating embryogenic cell suspensions (ECS) with colchicine and determining the ploidy of regenerated plants by flow cytometry. We have found that none of the plants regenerated from colchicine-treated ECS was mixoploid (chimeric). To optimise a protocol for the irradiation of ECS, several experiments were performed. Flow cytometric analysis of cell-cycle kinetics proved to be more informative than growth curves for the determination of the optimal timing of irradiation. The optimal irradiation dose was determined using three different parameters. Among them, the fresh weight gain and the reg...

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA6038204" target="_blank" >IAA6038204: Konstrukce knihovny DNA banánovníku klonované ve vektoru BAC (Bacterial artificial chromosome) pro fyzické mapování genomu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Banana Improvement: Cellular, Molecular Biology, and Induced Mutations

ISBN

1-57808-340-0

Počet stran výsledku

11

Strana od-do

33-43

Počet stran knihy

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Název nakladatele

Science Publishers, Inc

Místo vydání

Enfield

Kód UT WoS kapitoly

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