Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F09%3A00337706" target="_blank" >RIV/61389030:_____/09:00337706 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries

Popis výsledku v původním jazyce

In bread wheat comprehensive genome analysis is complicated by its very large genome (17 Gbp), polyploid nature and high repetitive sequence content. Using flow cytometry allows dissecting the wheat genome to individual chromosomes. Flow sorted chromosomes were used already to construct several grain BAC libraries including those from chromosome 3B and chromosome arms 1BS and 1RS. In the present work we compare the construction of the 3AS/3DS BAC libraries in respect to our new improved protocol which made two-sized selection feasible. A minimal tiling path of chromosome 3B has already been established. Construction of the 3AS- and 3DS-specific BAC libraries represent significant step toward completing BAC resources for the homoeologous chromosome group 3 of wheat and accelerate the development of sequence-ready physical contig maps and gene cloning, as well as comparative analyses aiming at revealing the genome changes accompanying the evolution of homoeologous chromosome group 3.

Název v anglickém jazyce

Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries

Popis výsledku anglicky

In bread wheat comprehensive genome analysis is complicated by its very large genome (17 Gbp), polyploid nature and high repetitive sequence content. Using flow cytometry allows dissecting the wheat genome to individual chromosomes. Flow sorted chromosomes were used already to construct several grain BAC libraries including those from chromosome 3B and chromosome arms 1BS and 1RS. In the present work we compare the construction of the 3AS/3DS BAC libraries in respect to our new improved protocol which made two-sized selection feasible. A minimal tiling path of chromosome 3B has already been established. Construction of the 3AS- and 3DS-specific BAC libraries represent significant step toward completing BAC resources for the homoeologous chromosome group 3 of wheat and accelerate the development of sequence-ready physical contig maps and gene cloning, as well as comparative analyses aiming at revealing the genome changes accompanying the evolution of homoeologous chromosome group 3.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Genetics & Breeding

ISSN

0394-9257

e-ISSN

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Svazek periodika

61

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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