Chromosome Genomics in the Triticeae

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Chromosome Genomics in the Triticeae

Popis výsledku v původním jazyce

Polyploidy and the presence of repeats make gene cloning and genome sequencing in the Triticeae extremely difficult, especially for tetraploid and hexaploid wheat. Chromosome genomics simplifies these tasks by targeting single chromosomes and chromosomearms, which represent only a few percent of the nuclear genomes. The advantages of this strategy over a whole-genome approach include the avoidance of problems due to the presence of homoeologs in wheat, reduction of work to manageable portions, cost efficiency, and an opportunity to structure collaborative projects where individual laboratories work on particular chromosomes. In this chapter, we describe how chromosomes and chromosome arms can be isolated by flow cytometric sorting and we review development of flow cytogenetics in the Triticeae. We then discuss various applications of flow-sorted chromosomes and assess the potential of chromosome genomics in the Triticeae.

Název v anglickém jazyce

Chromosome Genomics in the Triticeae

Popis výsledku anglicky

Polyploidy and the presence of repeats make gene cloning and genome sequencing in the Triticeae extremely difficult, especially for tetraploid and hexaploid wheat. Chromosome genomics simplifies these tasks by targeting single chromosomes and chromosomearms, which represent only a few percent of the nuclear genomes. The advantages of this strategy over a whole-genome approach include the avoidance of problems due to the presence of homoeologs in wheat, reduction of work to manageable portions, cost efficiency, and an opportunity to structure collaborative projects where individual laboratories work on particular chromosomes. In this chapter, we describe how chromosomes and chromosome arms can be isolated by flow cytometric sorting and we review development of flow cytogenetics in the Triticeae. We then discuss various applications of flow-sorted chromosomes and assess the potential of chromosome genomics in the Triticeae.

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Genetics and Genomics of the Triticeae

ISBN

978-0-387-77488-6

Počet stran výsledku

32

Strana od-do

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Počet stran knihy

757

Název nakladatele

Springer

Místo vydání

New York

Kód UT WoS kapitoly

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