SNP Discovery for mapping alien introgressions in wheat
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00429596" target="_blank" >RIV/61389030:_____/14:00429596 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1186/1471-2164-15-273" target="_blank" >http://dx.doi.org/10.1186/1471-2164-15-273</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/1471-2164-15-273" target="_blank" >10.1186/1471-2164-15-273</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
SNP Discovery for mapping alien introgressions in wheat
Popis výsledku v původním jazyce
The short arm of chromosome 5M(g) of Ae. geniculata Roth (syn. Ae. ovata L.; 2n = 4x = 28, (UUMMg)-U-g-M-g-M-g) was flow-sorted from a wheat line in which it is maintained as a telocentric chromosome. DNA of the sorted arm was amplified and sequenced using an Illumina Hiseq 2000 with similar to 45x coverage. The sequence data was used for SNP discovery against wheat homoeologous group-5 assemblies. A total of 2,178 unique, 5M(g)S-specific SNPs were discovered. Randomly selected samples of 59 5M(g)S-specific SNPs were tested (44 by KASPar assay and 15 by Sanger sequencing) and 84% were validated. Of the selected SNPs, 97% mapped to a chromosome 5M(g) addition to wheat (the source of t5M(g)S), and 94% to 5M(g) introgressed from a different accession of Ae. geniculata substituting for chromosome 5D of wheat. The validated SNPs also identified chromosome segments of 5M(g)S origin in a set of T5D-5M(g) translocation lines; eight SNPs (25%) mapped to TA5601 [T5DL . 5DS-5M(g)S(0.75)] and thre
Název v anglickém jazyce
SNP Discovery for mapping alien introgressions in wheat
Popis výsledku anglicky
The short arm of chromosome 5M(g) of Ae. geniculata Roth (syn. Ae. ovata L.; 2n = 4x = 28, (UUMMg)-U-g-M-g-M-g) was flow-sorted from a wheat line in which it is maintained as a telocentric chromosome. DNA of the sorted arm was amplified and sequenced using an Illumina Hiseq 2000 with similar to 45x coverage. The sequence data was used for SNP discovery against wheat homoeologous group-5 assemblies. A total of 2,178 unique, 5M(g)S-specific SNPs were discovered. Randomly selected samples of 59 5M(g)S-specific SNPs were tested (44 by KASPar assay and 15 by Sanger sequencing) and 84% were validated. Of the selected SNPs, 97% mapped to a chromosome 5M(g) addition to wheat (the source of t5M(g)S), and 94% to 5M(g) introgressed from a different accession of Ae. geniculata substituting for chromosome 5D of wheat. The validated SNPs also identified chromosome segments of 5M(g)S origin in a set of T5D-5M(g) translocation lines; eight SNPs (25%) mapped to TA5601 [T5DL . 5DS-5M(g)S(0.75)] and thre
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
B M C Genomics
ISSN
1471-2164
e-ISSN
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Svazek periodika
15
Číslo periodika v rámci svazku
APR 10
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
11
Strana od-do
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Kód UT WoS článku
000334958900001
EID výsledku v databázi Scopus
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