Whole-genome profiling and shotgun sequencing delivers an anchored, gene-decorated, physical map assembly of bread wheat chromosome 6A

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00432116" target="_blank" >RIV/61389030:_____/14:00432116 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/tpj.12550" target="_blank" >http://dx.doi.org/10.1111/tpj.12550</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tpj.12550" target="_blank" >10.1111/tpj.12550</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Whole-genome profiling and shotgun sequencing delivers an anchored, gene-decorated, physical map assembly of bread wheat chromosome 6A

Popis výsledku v původním jazyce

Bread wheat (Triticum aestivum L.) is the most important staple food crop for 35% of the world's population. International efforts are underway to facilitate an increase in wheat production, of which the International Wheat Genome Sequencing Consortium (IWGSC) plays an important role. As part of this effort, we have developed a sequence-based physical map of wheat chromosome 6A using whole-genome profiling (WGP (TM)). The bacterial artificial chromosome (BAC) contig assembly tools FINGERPRINTED CONTIG (FPC) and LINEAR TOPOLOGICAL CONTIG (LTC) were used and their contig assemblies were compared. A detailed investigation of the contigs structure revealed that LTC created a highly robust assembly compared with those formed by FPC. The LTC assemblies contained 1217 contigs for the short arm and 1113 contigs for the long arm, with an L-50 of 1 Mb. To facilitate in silico anchoring, WGP (TM) tags underlying BAC contigs were extended by wheat and wheat progenitor genome sequence information.

Název v anglickém jazyce

Whole-genome profiling and shotgun sequencing delivers an anchored, gene-decorated, physical map assembly of bread wheat chromosome 6A

Popis výsledku anglicky

Bread wheat (Triticum aestivum L.) is the most important staple food crop for 35% of the world's population. International efforts are underway to facilitate an increase in wheat production, of which the International Wheat Genome Sequencing Consortium (IWGSC) plays an important role. As part of this effort, we have developed a sequence-based physical map of wheat chromosome 6A using whole-genome profiling (WGP (TM)). The bacterial artificial chromosome (BAC) contig assembly tools FINGERPRINTED CONTIG (FPC) and LINEAR TOPOLOGICAL CONTIG (LTC) were used and their contig assemblies were compared. A detailed investigation of the contigs structure revealed that LTC created a highly robust assembly compared with those formed by FPC. The LTC assemblies contained 1217 contigs for the short arm and 1113 contigs for the long arm, with an L-50 of 1 Mb. To facilitate in silico anchoring, WGP (TM) tags underlying BAC contigs were extended by wheat and wheat progenitor genome sequence information.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Journal

ISSN

0960-7412

e-ISSN

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Svazek periodika

79

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

334-347

Kód UT WoS článku

000339439200013

EID výsledku v databázi Scopus

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