Transposable Element Junctions in Marker Development and Genomic Characterization of Barley

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00433526" target="_blank" >RIV/61389030:_____/14:00433526 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3835/plantgenome2013.10.0036" target="_blank" >http://dx.doi.org/10.3835/plantgenome2013.10.0036</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3835/plantgenome2013.10.0036" target="_blank" >10.3835/plantgenome2013.10.0036</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transposable Element Junctions in Marker Development and Genomic Characterization of Barley

Popis výsledku v původním jazyce

Barley is a model plant in genomic studies of Triticeae species. However, barley's large genome size and high repetitive sequence content complicate the whole-genome sequencing. The majority of the barley genome is composed of transposable elements (TEs). In this study, TE repeat junctions (RJs) were used to develop a large-scale molecular marker platform, as a prerequisite to genome assembly. A total of 10.22 Gb of barley nonassembled 454 sequencing data were screened with RJPrimers pipeline. In total,9,881,561 TE junctions were identified. From detected RJs, 400,538 polymerase chain reaction (PCR)-based RJ markers (RJMs) were designed across the genome, with an average of 39 markers/Mb. The utility of designed markers was tested using a random subset of RJMs. Over 94% of the markers successfully amplified amplicons, among which similar to 90% were genome specific. In addition to marker design, identified RJs were utilized to detect 1190,885 TEs across the genome. In gene-poor region

Název v anglickém jazyce

Transposable Element Junctions in Marker Development and Genomic Characterization of Barley

Popis výsledku anglicky

Barley is a model plant in genomic studies of Triticeae species. However, barley's large genome size and high repetitive sequence content complicate the whole-genome sequencing. The majority of the barley genome is composed of transposable elements (TEs). In this study, TE repeat junctions (RJs) were used to develop a large-scale molecular marker platform, as a prerequisite to genome assembly. A total of 10.22 Gb of barley nonassembled 454 sequencing data were screened with RJPrimers pipeline. In total,9,881,561 TE junctions were identified. From detected RJs, 400,538 polymerase chain reaction (PCR)-based RJ markers (RJMs) were designed across the genome, with an average of 39 markers/Mb. The utility of designed markers was tested using a random subset of RJMs. Over 94% of the markers successfully amplified amplicons, among which similar to 90% were genome specific. In addition to marker design, identified RJs were utilized to detect 1190,885 TEs across the genome. In gene-poor region

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Genome

ISSN

1940-3372

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000338833800008

EID výsledku v databázi Scopus

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