Identifying new sex-linked genes through BAC sequencing in the dioecious plant Silene latifolia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00451395" target="_blank" >RIV/61389030:_____/15:00451395 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/15:00447664

Výsledek na webu

<a href="http://dx.doi.org/10.1186/s12864-015-1698-7" target="_blank" >http://dx.doi.org/10.1186/s12864-015-1698-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-015-1698-7" target="_blank" >10.1186/s12864-015-1698-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identifying new sex-linked genes through BAC sequencing in the dioecious plant Silene latifolia

Popis výsledku v původním jazyce

Silene latifolia represents one of the best-studied plant sex chromosome systems. A new approach using RNA-seq data has recently identified hundreds of new sex-linked genes in this species. However, this approach is expected to miss genes that are eithernot expressed or are expressed at low levels in the tissue(s) used for RNA-seq. Therefore other independent approaches are needed to discover such sex-linked genes. Here we used 10 well-characterized S. latifolia sex-linked genes and their homologs in Silene vulgaris, a species without sex chromosomes, to screen BAC libraries of both species. We isolated and sequenced 4 Mb of BAC clones of S. latifolia X and Y and S. vulgaris genomic regions, which yielded 59 new sex-linked genes (with S. vulgaris homologs for some of them). We assembled sequences that we believe represent the tip of the Xq arm. These sequences are clearly not pseudoautosomal, so we infer that the S. latifolia X has a single pseudoautosomal region (PAR) on the Xp arm.

Název v anglickém jazyce

Identifying new sex-linked genes through BAC sequencing in the dioecious plant Silene latifolia

Popis výsledku anglicky

Silene latifolia represents one of the best-studied plant sex chromosome systems. A new approach using RNA-seq data has recently identified hundreds of new sex-linked genes in this species. However, this approach is expected to miss genes that are eithernot expressed or are expressed at low levels in the tissue(s) used for RNA-seq. Therefore other independent approaches are needed to discover such sex-linked genes. Here we used 10 well-characterized S. latifolia sex-linked genes and their homologs in Silene vulgaris, a species without sex chromosomes, to screen BAC libraries of both species. We isolated and sequenced 4 Mb of BAC clones of S. latifolia X and Y and S. vulgaris genomic regions, which yielded 59 new sex-linked genes (with S. vulgaris homologs for some of them). We assembled sequences that we believe represent the tip of the Xq arm. These sequences are clearly not pseudoautosomal, so we infer that the S. latifolia X has a single pseudoautosomal region (PAR) on the Xp arm.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP501%2F12%2F2220" target="_blank" >GAP501/12/2220: Evoluce pohlavních chromozomů - chromozomálně specifická genomika u rodu Silene</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

B M C Genomics

ISSN

1471-2164

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

JUL 25

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000358638500001

EID výsledku v databázi Scopus

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