Sequencing of Wheat Chromosome 6B: Toward Functional Genomics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00451909" target="_blank" >RIV/61389030:_____/15:00451909 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-4-431-55675-6_12" target="_blank" >http://dx.doi.org/10.1007/978-4-431-55675-6_12</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-4-431-55675-6_12" target="_blank" >10.1007/978-4-431-55675-6_12</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sequencing of Wheat Chromosome 6B: Toward Functional Genomics

Popis výsledku v původním jazyce

International Wheat Genome Sequencing Consortium (IWGSC) decided to adopt the strategy of chromosome sorting and short read assembly to overcome difficulties of wheat genome sequencing derived from the hexaploid status, the large genome size (about 17 Gb) and high repeat contents (more than 80 %). Our Japanese group was responsible for the sequencing of wheat chromosome 6B. Using DNAs from the flow-sorted chromosome arms, we conducted whole-chromosome shotgun sequencing of chromosome 6B. We sequenced more than 12 million reads obtained from the short and long arms by GS-FLX Titanium, and assembled contigs of 235 Mb for 6BS and 273 Mb for 6BL were generated by GS assembler 2.7 (Roche). These assemblies cover 56.6 % and 54.9 % of estimated sizes of 6BS (415 Mb) and 6BL (498 Mb), respectively. We annotated repetitive regions covering more than 80 % of contigs, 4,798 possible expressed loci, and various kinds of RNA genes using our annotation pipeline. We also found the evolutionary conser

Název v anglickém jazyce

Sequencing of Wheat Chromosome 6B: Toward Functional Genomics

Popis výsledku anglicky

International Wheat Genome Sequencing Consortium (IWGSC) decided to adopt the strategy of chromosome sorting and short read assembly to overcome difficulties of wheat genome sequencing derived from the hexaploid status, the large genome size (about 17 Gb) and high repeat contents (more than 80 %). Our Japanese group was responsible for the sequencing of wheat chromosome 6B. Using DNAs from the flow-sorted chromosome arms, we conducted whole-chromosome shotgun sequencing of chromosome 6B. We sequenced more than 12 million reads obtained from the short and long arms by GS-FLX Titanium, and assembled contigs of 235 Mb for 6BS and 273 Mb for 6BL were generated by GS assembler 2.7 (Roche). These assemblies cover 56.6 % and 54.9 % of estimated sizes of 6BS (415 Mb) and 6BL (498 Mb), respectively. We annotated repetitive regions covering more than 80 % of contigs, 4,798 possible expressed loci, and various kinds of RNA genes using our annotation pipeline. We also found the evolutionary conser

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Advances in Wheat Genetics: From Genome to Field

ISBN

978-4-431-55674-9

Počet stran výsledku

6

Strana od-do

111-116

Počet stran knihy

445

Název nakladatele

Springer

Místo vydání

Verlag

Kód UT WoS kapitoly

—