Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00474824" target="_blank" >RIV/61389030:_____/17:00474824 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/17:72094

Výsledek na webu

<a href="http://dx.doi.org/10.1080/09540105.2016.1272554" target="_blank" >http://dx.doi.org/10.1080/09540105.2016.1272554</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/09540105.2016.1272554" target="_blank" >10.1080/09540105.2016.1272554</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor

Popis výsledku v původním jazyce

Myrica rubra Sieb. et Zucc. is a valuable fruit tree that is used in Chinese, Japanese and Taiwanese traditional medicine. We investigated the anti-inflammatory activity of M. rubra leaves extracted with four different solvents. Total phenolics were determined using the Folin-Ciocalteu method. Extracts were investigated for their inhibitory activity toward the pro-inflammatory enzymes cyclooxygenase-1 and2 (COX-1, COX-2) and 5-lipoxygenase (5-LOX). The ethanol extract of M. rubra leaves demonstrated a strong inhibition of prostaglandin E-2 (PGE(2)) biosynthesis catalyzed by both COX-1 (93.42%) and COX-2 (75.71%) and leukotriene B-4 (LTB4) formation catalyzed by 5-LOX (82.72%). Further we identified selective COX-1 inhibition by the n-butanol and aqueous fractions of the ethanol extract (with an IC50 for COX-1 inhibition of 1.07 and 0.71 mu gmL(-1) (,) respectively) and dual 5-LOX/COX inhibition by the ethyl acetate fraction (with an IC50 of 3.29 for COX-1, 2.54 for COX-2 and 8.30 mu gmL(-1) for 5-LOX).

Název v anglickém jazyce

Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor

Popis výsledku anglicky

Myrica rubra Sieb. et Zucc. is a valuable fruit tree that is used in Chinese, Japanese and Taiwanese traditional medicine. We investigated the anti-inflammatory activity of M. rubra leaves extracted with four different solvents. Total phenolics were determined using the Folin-Ciocalteu method. Extracts were investigated for their inhibitory activity toward the pro-inflammatory enzymes cyclooxygenase-1 and2 (COX-1, COX-2) and 5-lipoxygenase (5-LOX). The ethanol extract of M. rubra leaves demonstrated a strong inhibition of prostaglandin E-2 (PGE(2)) biosynthesis catalyzed by both COX-1 (93.42%) and COX-2 (75.71%) and leukotriene B-4 (LTB4) formation catalyzed by 5-LOX (82.72%). Further we identified selective COX-1 inhibition by the n-butanol and aqueous fractions of the ethanol extract (with an IC50 for COX-1 inhibition of 1.07 and 0.71 mu gmL(-1) (,) respectively) and dual 5-LOX/COX inhibition by the ethyl acetate fraction (with an IC50 of 3.29 for COX-1, 2.54 for COX-2 and 8.30 mu gmL(-1) for 5-LOX).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30107 - Medicinal chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food and Agricultural Immunology

ISSN

0954-0105

e-ISSN

—

Svazek periodika

28

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

343-353

Kód UT WoS článku

000394647700015

EID výsledku v databázi Scopus

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