De novo transcriptome assembly of heavy metal tolerant Silene dioica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00475870" target="_blank" >RIV/61389030:_____/17:00475870 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/17:00507080

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.gdata.2017.01.004" target="_blank" >http://dx.doi.org/10.1016/j.gdata.2017.01.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.gdata.2017.01.004" target="_blank" >10.1016/j.gdata.2017.01.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

De novo transcriptome assembly of heavy metal tolerant Silene dioica

Popis výsledku v původním jazyce

Silene dioica is a dioecious plant of the family Caryophyllaceae. In the present study, we used Illumina sequencing technology (MiSeq) to sequence, de novo assembly and annotate the transcriptomes of male and female copper tolerant S. dioica individuals. We sequenced the normalized mRNA of roots, shoots, flower buds and flowers for each sex. Raw reads of the transcriptome assembly project for S. dioica male and female individual have been deposited in NCBI's Sequence Read Archive (SRA) database with the accession number SRP094611. The Trinity and Detonate program was used to de novo assembly 92,347 transcripts for male and 94,757 transcripts for female transcriptome. The assembled transcriptome sequences for S. dioica male and female individuals can be accessed at NCBI with the following accession numbers: GFCG00000000 (male), GFCH00000000 (female). The obtained transcriptomic data will be useful for further studies focusing on copper tolerance, comparative transcriptome analysis with other Silene species and sex chromosomes evolution.

Název v anglickém jazyce

De novo transcriptome assembly of heavy metal tolerant Silene dioica

Popis výsledku anglicky

Silene dioica is a dioecious plant of the family Caryophyllaceae. In the present study, we used Illumina sequencing technology (MiSeq) to sequence, de novo assembly and annotate the transcriptomes of male and female copper tolerant S. dioica individuals. We sequenced the normalized mRNA of roots, shoots, flower buds and flowers for each sex. Raw reads of the transcriptome assembly project for S. dioica male and female individual have been deposited in NCBI's Sequence Read Archive (SRA) database with the accession number SRP094611. The Trinity and Detonate program was used to de novo assembly 92,347 transcripts for male and 94,757 transcripts for female transcriptome. The assembled transcriptome sequences for S. dioica male and female individuals can be accessed at NCBI with the following accession numbers: GFCG00000000 (male), GFCH00000000 (female). The obtained transcriptomic data will be useful for further studies focusing on copper tolerance, comparative transcriptome analysis with other Silene species and sex chromosomes evolution.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GP13-34962P" target="_blank" >GP13-34962P: Silenka dvoudomá jako modelová rostlina pro studium tolerance k těžkým kovům</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

GENOMICS DATA

ISSN

2213-5960

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

2

Strana od-do

118-119

Kód UT WoS článku

000395325800030

EID výsledku v databázi Scopus

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