Changes in plasma membrane aquaporin gene expression under osmotic stress and blue light in tomato

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00487922" target="_blank" >RIV/61389030:_____/18:00487922 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/18:73591146

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11738-017-2602-7" target="_blank" >http://dx.doi.org/10.1007/s11738-017-2602-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11738-017-2602-7" target="_blank" >10.1007/s11738-017-2602-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Changes in plasma membrane aquaporin gene expression under osmotic stress and blue light in tomato

Popis výsledku v původním jazyce

Divergent abiotic stresses induce osmotic stress on plant cells resulting in an imbalance in water homeostasis which is preserved by aquaporins. Since the plasma membrane aquaporins (PIPs) were shown to be involved in seed development and responses to abiotic stresses, we focused on determining the contribution of mannitol-induced osmotic stress, blue light (BL), and 7B-1 mutation to their gene expression in tomato (Solanum lycopersicum L.) seeds. To assess that, we used a quantitative RT-PCR to determine the expression profiles of genes encoding PIPs. Subsequently, a multiple linear regression analysis was used to evaluate the impact of studied stressors (mannitol and BL) and 7B-1 mutation on PIP gene expressions. We found that mannitol-induced osmotic stress and 7B-1 mutation (conferring the lower responsiveness to osmotic stress-and BL-induced inhibition of seed germination) decreased expression of PIP1, 3, PIP2, 3 and PIP1, 2, PIP2, 1 genes, respectively. This might be a way to retain water for radicle elongation and seed germination under the stress conditions. Interestingly, the expression of PIP1, 3 gene was downregulated not only by osmotic stress, but also by BL. Altogether, our data indicate the existence of a link between osmotic stress and BL signalling and the involvement of the 7B-1 mutation in this crosstalk.

Název v anglickém jazyce

Changes in plasma membrane aquaporin gene expression under osmotic stress and blue light in tomato

Popis výsledku anglicky

Divergent abiotic stresses induce osmotic stress on plant cells resulting in an imbalance in water homeostasis which is preserved by aquaporins. Since the plasma membrane aquaporins (PIPs) were shown to be involved in seed development and responses to abiotic stresses, we focused on determining the contribution of mannitol-induced osmotic stress, blue light (BL), and 7B-1 mutation to their gene expression in tomato (Solanum lycopersicum L.) seeds. To assess that, we used a quantitative RT-PCR to determine the expression profiles of genes encoding PIPs. Subsequently, a multiple linear regression analysis was used to evaluate the impact of studied stressors (mannitol and BL) and 7B-1 mutation on PIP gene expressions. We found that mannitol-induced osmotic stress and 7B-1 mutation (conferring the lower responsiveness to osmotic stress-and BL-induced inhibition of seed germination) decreased expression of PIP1, 3, PIP2, 3 and PIP1, 2, PIP2, 1 genes, respectively. This might be a way to retain water for radicle elongation and seed germination under the stress conditions. Interestingly, the expression of PIP1, 3 gene was downregulated not only by osmotic stress, but also by BL. Altogether, our data indicate the existence of a link between osmotic stress and BL signalling and the involvement of the 7B-1 mutation in this crosstalk.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000424497300006

EID výsledku v databázi Scopus

—