Expression of genes encoding PIP aquaporins in tomato seeds exposed to blue radiation and mercury

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00504627" target="_blank" >RIV/61389030:_____/19:00504627 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/19:73597164

Výsledek na webu

<a href="http://doi.org/10.32615/bp.2019.001" target="_blank" >http://doi.org/10.32615/bp.2019.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.32615/bp.2019.001" target="_blank" >10.32615/bp.2019.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression of genes encoding PIP aquaporins in tomato seeds exposed to blue radiation and mercury

Popis výsledku v původním jazyce

Aquaporins control the specific transport of water and some other small molecules across membranes and are involved in various physiological processes. Plasma membrane aquaporins (PIPs) were shown to play an important role during tomato seed development. Therefore, we were interested in the participation of PIPs in seed germination and early seedling growth of wild type (WT) and the 7B-1 mutant, affected by blue light responses. We characterised the expression patterns of PIP-type aquaporin genes in these lines during different phases of seed germination and seedling growth after HgCl2 (an aquaporin blocker) treatment. Further, we investigated whether blue radiation (BL) was involved in the regulation of these processes. Our experiments showed that 7B-1 mutant seed germination and root elongation are less responsive to HgCl2 compared to WT. In both WT and 7B-1 mutant seeds, BL modulates the expression of PIP1,1 (upregulation) and PIP1,3 (downregulation) aquaporin isoforms. The PIP1,3 gene is downregulated not only by BL but also by HgCl2 with a stronger effect in WT seeds. Thus, we show that BL can alter PIPs gene expression during tomato seed germination and seedling growth and that the 7B-1 mutation reduced the responsiveness to mercury blockage of aquaporins. Altogether, our data indicate that PIP aquaporins participate in tomato seed germination and radicle elongation and that the 7B-1 mutation and BL have an impact on these processes.

Název v anglickém jazyce

Expression of genes encoding PIP aquaporins in tomato seeds exposed to blue radiation and mercury

Popis výsledku anglicky

Aquaporins control the specific transport of water and some other small molecules across membranes and are involved in various physiological processes. Plasma membrane aquaporins (PIPs) were shown to play an important role during tomato seed development. Therefore, we were interested in the participation of PIPs in seed germination and early seedling growth of wild type (WT) and the 7B-1 mutant, affected by blue light responses. We characterised the expression patterns of PIP-type aquaporin genes in these lines during different phases of seed germination and seedling growth after HgCl2 (an aquaporin blocker) treatment. Further, we investigated whether blue radiation (BL) was involved in the regulation of these processes. Our experiments showed that 7B-1 mutant seed germination and root elongation are less responsive to HgCl2 compared to WT. In both WT and 7B-1 mutant seeds, BL modulates the expression of PIP1,1 (upregulation) and PIP1,3 (downregulation) aquaporin isoforms. The PIP1,3 gene is downregulated not only by BL but also by HgCl2 with a stronger effect in WT seeds. Thus, we show that BL can alter PIPs gene expression during tomato seed germination and seedling growth and that the 7B-1 mutation reduced the responsiveness to mercury blockage of aquaporins. Altogether, our data indicate that PIP aquaporins participate in tomato seed germination and radicle elongation and that the 7B-1 mutation and BL have an impact on these processes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biologia Plantarum

ISSN

0006-3134

e-ISSN

—

Svazek periodika

63

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000466354600003

EID výsledku v databázi Scopus

2-s2.0-85067689930