Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00532865" target="_blank" >RIV/61389030:_____/20:00532865 - isvavai.cz</a>
Výsledek na webu
<a href="http://doi.org/10.1016/j.envexpbot.2020.104236" target="_blank" >http://doi.org/10.1016/j.envexpbot.2020.104236</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.envexpbot.2020.104236" target="_blank" >10.1016/j.envexpbot.2020.104236</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema
Popis výsledku v původním jazyce
One of the early reactions of plants to high levels of NaCl is the generation of reactive oxygen species (ROS), which is critical for stress signaling, Na+ extrusion, oxidative damage and programmed cell death (PCD) under salt stress. Using moss Physcomitrella patens protonema as a model system, we have tested whether ROS generation and DNA degradation can be induced by salinity in Bryophyta. We have shown with dihydroethidium in vivo test that toxic NaCl concentrations significantly stimulate ROS production. The pharmacological analysis carried out using superoxide dismutase, catalase, dimethyl sulfoxide, thiourea and spermine outlines the superoxide and hydroxyl radicals as major NaCl-induced species. Using Comet assay, we have found that NaCl affects the DNA stability in ROS-dependent manner, increasing both single and double strand DNA breaks (SSBs and DSBs, respectively). Surprisingly, DSBs were induced by NaCl to a lesser extent than SSBs. This is indicative of an unexpected and sophisticated action of NaCl on moss genome including DSBs through PCD and SSBs caused by hydroxyl radical-induced DNA oxidation or transposon rearrangement. We also report a relatively high sensitivity of Physcomitrella patens protonema to NaCl compared to later developmental stages of this moss, suggesting that dividing cells are more sensitive to NaCl than other tissues.
Název v anglickém jazyce
Salt stress triggers generation of oxygen free radicals and DNA breaks in Physcomitrella patens protonema
Popis výsledku anglicky
One of the early reactions of plants to high levels of NaCl is the generation of reactive oxygen species (ROS), which is critical for stress signaling, Na+ extrusion, oxidative damage and programmed cell death (PCD) under salt stress. Using moss Physcomitrella patens protonema as a model system, we have tested whether ROS generation and DNA degradation can be induced by salinity in Bryophyta. We have shown with dihydroethidium in vivo test that toxic NaCl concentrations significantly stimulate ROS production. The pharmacological analysis carried out using superoxide dismutase, catalase, dimethyl sulfoxide, thiourea and spermine outlines the superoxide and hydroxyl radicals as major NaCl-induced species. Using Comet assay, we have found that NaCl affects the DNA stability in ROS-dependent manner, increasing both single and double strand DNA breaks (SSBs and DSBs, respectively). Surprisingly, DSBs were induced by NaCl to a lesser extent than SSBs. This is indicative of an unexpected and sophisticated action of NaCl on moss genome including DSBs through PCD and SSBs caused by hydroxyl radical-induced DNA oxidation or transposon rearrangement. We also report a relatively high sensitivity of Physcomitrella patens protonema to NaCl compared to later developmental stages of this moss, suggesting that dividing cells are more sensitive to NaCl than other tissues.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/LTC17047" target="_blank" >LTC17047: Využití rostlin při monitorování poškození lidské DNA</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Environmental and Experimental Botany
ISSN
0098-8472
e-ISSN
—
Svazek periodika
180
Číslo periodika v rámci svazku
December
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
11
Strana od-do
104236
Kód UT WoS článku
000579447700008
EID výsledku v databázi Scopus
2-s2.0-85090573884