Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00546297" target="_blank" >RIV/61389030:_____/21:00546297 - isvavai.cz</a>

Výsledek na webu

<a href="http://doi.org/10.1007/s11240-021-02012-7" target="_blank" >http://doi.org/10.1007/s11240-021-02012-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-021-02012-7" target="_blank" >10.1007/s11240-021-02012-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

Popis výsledku v původním jazyce

The effectiveness of microspore embryogenesis (ME) is determined by a complex network of internal and environmental factors. In the present study on triticale and barley, strong positive correlation (r = 0.85) between the generation of hydrogen peroxide (H2O2) and ME effectiveness confirmed the important role of reactive oxygen species in microspore reprogramming. However, for high effectiveness of ME induction, intensive H2O2 generation had to be associated with high activity of antioxidative enzymes, superoxide dismutase and catalase. The strong seasonal effect on the physiological status of microspores revealed in the study suggests a kind of ‘biological clock’ controlling plant reproduction, crucial for microspore viability and embryogenic potential. Although the effect of various modifications of ME-inducing stress tiller pre-treatment was determined mainly by the physiological condition of microspores, at higher stress intensity positive effects induced by antioxidant molecules—reduced glutathione and its precursor, l-2-oxothiazolidine-4-carboxylic acid—were observed. High level of variation in the response to ME-inducing stress tiller pre-treatment was also revealed between the two DH lines of triticale and two cultivars of barley and among microspores isolated from subsequently developed spikes.

Název v anglickém jazyce

Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

Popis výsledku anglicky

The effectiveness of microspore embryogenesis (ME) is determined by a complex network of internal and environmental factors. In the present study on triticale and barley, strong positive correlation (r = 0.85) between the generation of hydrogen peroxide (H2O2) and ME effectiveness confirmed the important role of reactive oxygen species in microspore reprogramming. However, for high effectiveness of ME induction, intensive H2O2 generation had to be associated with high activity of antioxidative enzymes, superoxide dismutase and catalase. The strong seasonal effect on the physiological status of microspores revealed in the study suggests a kind of ‘biological clock’ controlling plant reproduction, crucial for microspore viability and embryogenic potential. Although the effect of various modifications of ME-inducing stress tiller pre-treatment was determined mainly by the physiological condition of microspores, at higher stress intensity positive effects induced by antioxidant molecules—reduced glutathione and its precursor, l-2-oxothiazolidine-4-carboxylic acid—were observed. High level of variation in the response to ME-inducing stress tiller pre-treatment was also revealed between the two DH lines of triticale and two cultivars of barley and among microspores isolated from subsequently developed spikes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Svazek periodika

145

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

20

Strana od-do

347-366

Kód UT WoS článku

000612256400001

EID výsledku v databázi Scopus

2-s2.0-85099989576