Hormonal homeostasis associated with effective induction of triticale microspore embryogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00572850" target="_blank" >RIV/61389030:_____/23:00572850 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s11240-022-02433-y" target="_blank" >https://doi.org/10.1007/s11240-022-02433-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-022-02433-y" target="_blank" >10.1007/s11240-022-02433-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hormonal homeostasis associated with effective induction of triticale microspore embryogenesis

Popis výsledku v původním jazyce

The endogenous level of cytokinins (CKs) and auxins (Auxs) was analyzed in isolated microspores and ovaries of two doubled haploid (DH) lines of triticale (× Triticosecale Wittm.) to better understand the mechanism of microspore embryogenesis (ME)—the most efficient and widely applied method of producing DHs. The responsiveness of the studied lines to ME significantly varied. ME was induced by pre-treating tillers with low temperature (4 °C for 3 weeks) alone or in combination with synthetic auxin (2,4-D), auxin inhibitor (PCIB) or melatonin (MEL) applied for 4 days before microspore isolation. Hormonal profile analyses, accompanied by ME effectiveness evaluation confirmed the multi-level crosstalk of Auxs/CKs and the specific hormonal homeostasis required for effective microspore reprogramming. It was found that triticale microspores contained mainly cis zeatin derivatives: cis-zeatin-O-glucoside (cZOG), cis-zeatin riboside (cZR) and cis-zeatin (cZ), as well as indole-3-acetic acid (IAA) and IAA-aspartate (IAAsp). Increased ME efficiency was associated with higher contents of most of the identified CKs and Auxs, as well as the higher active Aux/active CK ratio. Trans CK isoforms were detected only in ovaries, confirming their importance as a source of bioactive molecules stimulating embryogenic development. Two of the pre-treatments tested: 12.5 μM PCIB and 50 μM MEL decreased the active Aux/active CK ratio, which was accompanied by an increase in the regeneration efficiency.

Název v anglickém jazyce

Hormonal homeostasis associated with effective induction of triticale microspore embryogenesis

Popis výsledku anglicky

The endogenous level of cytokinins (CKs) and auxins (Auxs) was analyzed in isolated microspores and ovaries of two doubled haploid (DH) lines of triticale (× Triticosecale Wittm.) to better understand the mechanism of microspore embryogenesis (ME)—the most efficient and widely applied method of producing DHs. The responsiveness of the studied lines to ME significantly varied. ME was induced by pre-treating tillers with low temperature (4 °C for 3 weeks) alone or in combination with synthetic auxin (2,4-D), auxin inhibitor (PCIB) or melatonin (MEL) applied for 4 days before microspore isolation. Hormonal profile analyses, accompanied by ME effectiveness evaluation confirmed the multi-level crosstalk of Auxs/CKs and the specific hormonal homeostasis required for effective microspore reprogramming. It was found that triticale microspores contained mainly cis zeatin derivatives: cis-zeatin-O-glucoside (cZOG), cis-zeatin riboside (cZR) and cis-zeatin (cZ), as well as indole-3-acetic acid (IAA) and IAA-aspartate (IAAsp). Increased ME efficiency was associated with higher contents of most of the identified CKs and Auxs, as well as the higher active Aux/active CK ratio. Trans CK isoforms were detected only in ovaries, confirming their importance as a source of bioactive molecules stimulating embryogenic development. Two of the pre-treatments tested: 12.5 μM PCIB and 50 μM MEL decreased the active Aux/active CK ratio, which was accompanied by an increase in the regeneration efficiency.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/EF16_019%2F0000738" target="_blank" >EF16_019/0000738: Centrum experimentální biologie rostlin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Svazek periodika

152

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

22

Strana od-do

583-604

Kód UT WoS článku

000904002900001

EID výsledku v databázi Scopus

2-s2.0-85144903682