Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CIOX proteins and cytokinin responses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00552834" target="_blank" >RIV/61389030:_____/21:00552834 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/21:73610743

Výsledek na webu

<a href="http://doi.org/10.1016/j.molp.2021.07.015" target="_blank" >http://doi.org/10.1016/j.molp.2021.07.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molp.2021.07.015" target="_blank" >10.1016/j.molp.2021.07.015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CIOX proteins and cytokinin responses

Popis výsledku v původním jazyce

Eukaryotic organisms are equipped with quality-control mechanisms that survey protein folding in the endoplasmic reticulum (ER) and remove non-native proteins by ER-associated degradation (ERAD). Recent research has shown that cytokinin-degrading CIOX proteins are subjected to ERAD during plant development. The mechanisms of plant ERAD, including the export of substrate proteins from the ER, are not fully understood, and the molecular components involved in the ERAD of CIOX are unknown. Here, we show that heavy metal-associated isoprenylated plant proteins (HIPPs) interact specifically with CIOX proteins synthesized in the ER and processed by ERAD. CIOX-HIPP protein complexes were detected at the ER as well as in the cytosol, suggesting that the complexes involve retrotranslocated CIOX protein species. Altered CIOX levels in HIPP-overexpressing and higher-order hipp mutant plants suggest that the studied HIPPs control the ERAD of CIOX. Deregulation of CIOX proteins caused corresponding changes in the cytokinin signaling activity and triggered typical morphological cytokinin responses. Notably, transcriptional repression of HIPP genes by cytokinin indicates a feedback regulatory mechanism of cytokinin homeostasis and signaling responses. Moreover, loss of function of HIPP genes constitutively activates the unfolded protein response and compromises the ER stress tolerance. Collectively, these results suggests that HIPPs represent novel functional components of plant ERAD.

Název v anglickém jazyce

Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CIOX proteins and cytokinin responses

Popis výsledku anglicky

Eukaryotic organisms are equipped with quality-control mechanisms that survey protein folding in the endoplasmic reticulum (ER) and remove non-native proteins by ER-associated degradation (ERAD). Recent research has shown that cytokinin-degrading CIOX proteins are subjected to ERAD during plant development. The mechanisms of plant ERAD, including the export of substrate proteins from the ER, are not fully understood, and the molecular components involved in the ERAD of CIOX are unknown. Here, we show that heavy metal-associated isoprenylated plant proteins (HIPPs) interact specifically with CIOX proteins synthesized in the ER and processed by ERAD. CIOX-HIPP protein complexes were detected at the ER as well as in the cytosol, suggesting that the complexes involve retrotranslocated CIOX protein species. Altered CIOX levels in HIPP-overexpressing and higher-order hipp mutant plants suggest that the studied HIPPs control the ERAD of CIOX. Deregulation of CIOX proteins caused corresponding changes in the cytokinin signaling activity and triggered typical morphological cytokinin responses. Notably, transcriptional repression of HIPP genes by cytokinin indicates a feedback regulatory mechanism of cytokinin homeostasis and signaling responses. Moreover, loss of function of HIPP genes constitutively activates the unfolded protein response and compromises the ER stress tolerance. Collectively, these results suggests that HIPPs represent novel functional components of plant ERAD.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Plant

ISSN

1674-2052

e-ISSN

1752-9867

Svazek periodika

14

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

1918-1934

Kód UT WoS článku

000715075800016

EID výsledku v databázi Scopus

2-s2.0-85114248476