Phytochemical, LC-MS Studies, and Assessment of Antioxidant, Anti-inflammatory, Hypoglycemic, Anti-Rheumatoid-arthritis Potential with Acmella paniculata: In-Silico and In-Vitro Appraisal

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00635922" target="_blank" >RIV/61389030:_____/25:00635922 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1002/slct.202405189" target="_blank" >https://doi.org/10.1002/slct.202405189</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/slct.202405189" target="_blank" >10.1002/slct.202405189</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phytochemical, LC-MS Studies, and Assessment of Antioxidant, Anti-inflammatory, Hypoglycemic, Anti-Rheumatoid-arthritis Potential with Acmella paniculata: In-Silico and In-Vitro Appraisal

Popis výsledku v původním jazyce

Acmella paniculata (AP), an ethnomedicinal plant, is valued for the management of pain, inflammation, arthritis, infection, diabetes and as tribal cuisine The study focused on validating AP against inflammatory pain, rheumatoid arthritis (RA), and hyperglycemia. Hydro-ethanolic extract of AP flower (HEFeAP) was prepared via maceration, followed by ultrasonication. Physicochemical characterization was done using Thin Layer Chromatography (TLC), Liquid Chromatography-Mass Spectrometry (LC-MS), Ultra-violet Spectroscopy (UV), and Fourier Transform Infrared Spectroscopy (FTIR). Major phytochemicals were assessed against inflammatory mediators using molecular docking and Molecular Dynamics (MD) simulations. The antioxidant potential of HEFeAP was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay (31.22 +/- 0.53 mu g mL-1) and hydrogen peroxide (H2O2) scavenging assay (40.36 +/- 0.47 mu g mL-1). The anti-inflammatory activity, protective role against RA, and anti-hyperglycaemic activities were evaluated via egg-albumin denaturation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay against human-fibroblast cells and alpha-amylase inhibition, showing IC50 of 27.61, 65.75, and 85.43 mu g mL-1, respectively. Tumor Necrosis Factor-alpha (TNF-alpha), and nitric-oxide (NO) levels were significantly downregulated in LPS-stimulated PBMC-derived macrophages (IC50 = 152 and 164.56 mu g mL-1). Phytochemical analysis revealed coumarins, flavonoids, polyphenols, steroids, and local-anaesthetic alkylamides. Active phytomolecules in HEFeAP exhibited strong and stable binding with inflammatory biomarkers. These findings suggest HEFeAP can be used as a potential candidate for inflammatory pain, RA, and diabetes.

Název v anglickém jazyce

Phytochemical, LC-MS Studies, and Assessment of Antioxidant, Anti-inflammatory, Hypoglycemic, Anti-Rheumatoid-arthritis Potential with Acmella paniculata: In-Silico and In-Vitro Appraisal

Popis výsledku anglicky

Acmella paniculata (AP), an ethnomedicinal plant, is valued for the management of pain, inflammation, arthritis, infection, diabetes and as tribal cuisine The study focused on validating AP against inflammatory pain, rheumatoid arthritis (RA), and hyperglycemia. Hydro-ethanolic extract of AP flower (HEFeAP) was prepared via maceration, followed by ultrasonication. Physicochemical characterization was done using Thin Layer Chromatography (TLC), Liquid Chromatography-Mass Spectrometry (LC-MS), Ultra-violet Spectroscopy (UV), and Fourier Transform Infrared Spectroscopy (FTIR). Major phytochemicals were assessed against inflammatory mediators using molecular docking and Molecular Dynamics (MD) simulations. The antioxidant potential of HEFeAP was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay (31.22 +/- 0.53 mu g mL-1) and hydrogen peroxide (H2O2) scavenging assay (40.36 +/- 0.47 mu g mL-1). The anti-inflammatory activity, protective role against RA, and anti-hyperglycaemic activities were evaluated via egg-albumin denaturation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay against human-fibroblast cells and alpha-amylase inhibition, showing IC50 of 27.61, 65.75, and 85.43 mu g mL-1, respectively. Tumor Necrosis Factor-alpha (TNF-alpha), and nitric-oxide (NO) levels were significantly downregulated in LPS-stimulated PBMC-derived macrophages (IC50 = 152 and 164.56 mu g mL-1). Phytochemical analysis revealed coumarins, flavonoids, polyphenols, steroids, and local-anaesthetic alkylamides. Active phytomolecules in HEFeAP exhibited strong and stable binding with inflammatory biomarkers. These findings suggest HEFeAP can be used as a potential candidate for inflammatory pain, RA, and diabetes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/GA23-05389S" target="_blank" >GA23-05389S: Nové CB2 a BChE modulátory proti Parkinsonově chorobě a souvisejícím patologiím</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemistrySelect

ISSN

2365-6549

e-ISSN

2365-6549

Svazek periodika

10

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

23

Strana od-do

e202405189

Kód UT WoS článku

001479588400001

EID výsledku v databázi Scopus

2-s2.0-105004211704