Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-kappa B and AP-1 Activities In Vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73604544" target="_blank" >RIV/61989592:15310/20:73604544 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/21/14/4897/htm" target="_blank" >https://www.mdpi.com/1422-0067/21/14/4897/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21144897" target="_blank" >10.3390/ijms21144897</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-kappa B and AP-1 Activities In Vitro

Popis výsledku v původním jazyce

Here we report the comprehensive characterization of the secondary metabolites from the leaves ofColebrookea oppositifoliaSmith, a species used as medicinal plant in the traditional medicine of Nepal. Phytochemical screening of bioactives was performed using an integrated LC-MS(n)and high resolution MS (Mass Spectrometry) approach. Forty-three compounds were tentatively identified, mainly aglyconic and glycosilated flavonoids and phenolic acids, as well as other bioactives such as coumarins and terpenes were detected. Furthermore, the NF-kappa B and AP-1 inhibitory activity ofC. oppositifoliaextract were evaluated, as well as its cytotoxicity against THP-1 cells, in order to assess the potential use of this herb as a source of anti-inflammatory and cytotoxic compounds. The results so far obtained indicate thatC. oppositifolialeaves extract could significantly reduce the viability of THP-1 cells (IC50= 6.2 +/- 1.2 mu g/mL), as well as the activation of both NF-kappa B and AP-1 at the concentration of 2 mu g/mL. Our results indicate that NepaleseC. oppositifoliais a valuable source of anti-inflammatory and cytotoxic compounds. The phytochemical composition reported here can partially justify the traditional uses ofC. oppositifoliain Nepal, especially in the treatment of inflammatory diseases, although further research will be needed to assess the full potential of this species.

Název v anglickém jazyce

Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-kappa B and AP-1 Activities In Vitro

Popis výsledku anglicky

Here we report the comprehensive characterization of the secondary metabolites from the leaves ofColebrookea oppositifoliaSmith, a species used as medicinal plant in the traditional medicine of Nepal. Phytochemical screening of bioactives was performed using an integrated LC-MS(n)and high resolution MS (Mass Spectrometry) approach. Forty-three compounds were tentatively identified, mainly aglyconic and glycosilated flavonoids and phenolic acids, as well as other bioactives such as coumarins and terpenes were detected. Furthermore, the NF-kappa B and AP-1 inhibitory activity ofC. oppositifoliaextract were evaluated, as well as its cytotoxicity against THP-1 cells, in order to assess the potential use of this herb as a source of anti-inflammatory and cytotoxic compounds. The results so far obtained indicate thatC. oppositifolialeaves extract could significantly reduce the viability of THP-1 cells (IC50= 6.2 +/- 1.2 mu g/mL), as well as the activation of both NF-kappa B and AP-1 at the concentration of 2 mu g/mL. Our results indicate that NepaleseC. oppositifoliais a valuable source of anti-inflammatory and cytotoxic compounds. The phytochemical composition reported here can partially justify the traditional uses ofC. oppositifoliain Nepal, especially in the treatment of inflammatory diseases, although further research will be needed to assess the full potential of this species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/LO1305" target="_blank" >LO1305: Rozvoj centra pokročilých technologií a materiálů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN

1422-0067

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

"4897-1"-"4897-15"

Kód UT WoS článku

000556487000001

EID výsledku v databázi Scopus

2-s2.0-85087741434