Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00637606" target="_blank" >RIV/61389030:_____/25:00637606 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1038/s41598-025-02900-4" target="_blank" >https://doi.org/10.1038/s41598-025-02900-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-02900-4" target="_blank" >10.1038/s41598-025-02900-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus

Popis výsledku v původním jazyce

Pleurotus is an edible mushroom that is consumed and cultivated worldwide. Pleurotus djamor var. fuscopruinosus is newly reported in Thailand, confirmed through morphological and phylogenetic analyses. Cultivation trials showed optimal mycelial growth on PDA, with sorghum supporting the highest spawn production. Sawdust was a suitable substrate, with fruiting bodies forming at 25-28 degrees C and 70-80% humidity, maturing within 4-5 days. Nutritional analysis (per 100 g dry weight) revealed 25.00 +/- 1.5% protein, 15.99 +/- 0.2% carbohydrates, 19.10 +/- 0.9% fiber, 7.59 +/- 1.0% ash, 2.07 +/- 0.5% fat, and 30.25 +/- 0.5% moisture. The 50% ethyl acetate extract exhibited the strongest antioxidant activity (IC50: 694.47 +/- 3.92 mu g/mL for DPPH, 652.92 +/- 2.53 mu g/mL for ABTS). The extract showed selective cytotoxicity against lung (A549, IC50 = 245.73 +/- 7.60 mu g/mL) and colorectal (SW480, IC50 = 382.03 +/- 4.55 mu g/mL) cancer cells but was less potent than doxorubicin (IC50 = 14.96 +/- 1.58 mu g/mL). Moderate cytotoxicity was observed in RAW 264.7 macrophages (IC50 = 213.08 +/- 4.08 mu g/mL). Antidiabetic potential was demonstrated through alpha-glucosidase inhibition, with the hot water extract (IC50 = 582.91 +/- 3.0 mu g/mL) showing the highest activity, followed by ethyl acetate (IC50 = 473.87 +/- 1.4 mu g/mL) and methanol (IC50 = 357.63 +/- 3.3 mu g/mL), comparable to acarbose (IC50 = 635.70 +/- 4.9 mu g/mL). Glucose consumption in 3T3-L1 adipocytes was enhanced (IC50 = 582.91 +/- 3.0 mu g/mL) but lower than metformin (IC50 = 99.58 +/- 0.59 mu g/mL). These results highlight P. djamor var. fuscopruinosus as a promising functional food with strong antioxidant, anticancer effects, and antidiabetic properties. Further studies should optimize cultivation, isolate bioactive compounds, assess safety, and validate therapeutic properties through in vivo studies.

Název v anglickém jazyce

Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus

Popis výsledku anglicky

Pleurotus is an edible mushroom that is consumed and cultivated worldwide. Pleurotus djamor var. fuscopruinosus is newly reported in Thailand, confirmed through morphological and phylogenetic analyses. Cultivation trials showed optimal mycelial growth on PDA, with sorghum supporting the highest spawn production. Sawdust was a suitable substrate, with fruiting bodies forming at 25-28 degrees C and 70-80% humidity, maturing within 4-5 days. Nutritional analysis (per 100 g dry weight) revealed 25.00 +/- 1.5% protein, 15.99 +/- 0.2% carbohydrates, 19.10 +/- 0.9% fiber, 7.59 +/- 1.0% ash, 2.07 +/- 0.5% fat, and 30.25 +/- 0.5% moisture. The 50% ethyl acetate extract exhibited the strongest antioxidant activity (IC50: 694.47 +/- 3.92 mu g/mL for DPPH, 652.92 +/- 2.53 mu g/mL for ABTS). The extract showed selective cytotoxicity against lung (A549, IC50 = 245.73 +/- 7.60 mu g/mL) and colorectal (SW480, IC50 = 382.03 +/- 4.55 mu g/mL) cancer cells but was less potent than doxorubicin (IC50 = 14.96 +/- 1.58 mu g/mL). Moderate cytotoxicity was observed in RAW 264.7 macrophages (IC50 = 213.08 +/- 4.08 mu g/mL). Antidiabetic potential was demonstrated through alpha-glucosidase inhibition, with the hot water extract (IC50 = 582.91 +/- 3.0 mu g/mL) showing the highest activity, followed by ethyl acetate (IC50 = 473.87 +/- 1.4 mu g/mL) and methanol (IC50 = 357.63 +/- 3.3 mu g/mL), comparable to acarbose (IC50 = 635.70 +/- 4.9 mu g/mL). Glucose consumption in 3T3-L1 adipocytes was enhanced (IC50 = 582.91 +/- 3.0 mu g/mL) but lower than metformin (IC50 = 99.58 +/- 0.59 mu g/mL). These results highlight P. djamor var. fuscopruinosus as a promising functional food with strong antioxidant, anticancer effects, and antidiabetic properties. Further studies should optimize cultivation, isolate bioactive compounds, assess safety, and validate therapeutic properties through in vivo studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

20

Strana od-do

18929

Kód UT WoS článku

001499347000029

EID výsledku v databázi Scopus

2-s2.0-105006932397