Analysis of DNA single-nucleotide polymorphism in maize inbreds with different degrees of sensitivity to head smut (Sporisorium reilianum)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00637639" target="_blank" >RIV/61389030:_____/25:00637639 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.15407/agrisp12.01.063" target="_blank" >https://doi.org/10.15407/agrisp12.01.063</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15407/agrisp12.01.063" target="_blank" >10.15407/agrisp12.01.063</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of DNA single-nucleotide polymorphism in maize inbreds with different degrees of sensitivity to head smut (Sporisorium reilianum)

Popis výsledku v původním jazyce

Aim. nSingle-nucleotide polymorphism analysis of maize inbreds with different rate of sensitivity to the head caused by Sporisorium reilianum, assessment of genetic relationships between them, and search for statistically significant associations between SNP markers and phenotypic manifestation of pathogen resistance. nMethods. nThe single-nucleotide polymorphism analysis using BDI-III panel with 384 SNP markers, statistical methods. Results. The SNP analysis of seven maize inbreds with different sensitivity to S. reilianum infection was out. The key indicators of single-nucleotide polymorphism markers with BDI-III panel were assessed: tion of dimorphic markers was 85.8 %, the average minor allele frequency was 0.2984, the fraction of dimorphic markers with the minor allele frequency >0.2 was 68.2 %, the average polymorphism information content was 0.2770. The main indicators of the maize inbreds were determined by SNP markers. It was established the average homozygosity of the inbreds was 98.5 %, the average gene diversity index was 0.1723, and age genetic distance was 0.4975. It was found the allelic state of SNP markers of the BDI-III panel-270C and BDI-III-292A, which is characteristic of maize inbreds resistant to head smut, as well as BDI-III-270A and BDI-III-292G for maize inbreds sensitive to head smut. The dendrogram of genetic relationships studied maize inbreds was drawn.nConclusions. nThe presence of statistically significant associations the allelic state of two SNP markers and the phenotypic manifestation of the maize resistance to head smut established. The random nature of the location of maize inbreds sensitive to head smut on the dendrogram genetic relationships was revealed. In the future, the identified single-nucleotide polymorphism markers, were associated with head smut resistance, will be verified on a larger number of maize genotypes of origin to confirm the possibility of their practical application.

Název v anglickém jazyce

Analysis of DNA single-nucleotide polymorphism in maize inbreds with different degrees of sensitivity to head smut (Sporisorium reilianum)

Popis výsledku anglicky

Aim. nSingle-nucleotide polymorphism analysis of maize inbreds with different rate of sensitivity to the head caused by Sporisorium reilianum, assessment of genetic relationships between them, and search for statistically significant associations between SNP markers and phenotypic manifestation of pathogen resistance. nMethods. nThe single-nucleotide polymorphism analysis using BDI-III panel with 384 SNP markers, statistical methods. Results. The SNP analysis of seven maize inbreds with different sensitivity to S. reilianum infection was out. The key indicators of single-nucleotide polymorphism markers with BDI-III panel were assessed: tion of dimorphic markers was 85.8 %, the average minor allele frequency was 0.2984, the fraction of dimorphic markers with the minor allele frequency >0.2 was 68.2 %, the average polymorphism information content was 0.2770. The main indicators of the maize inbreds were determined by SNP markers. It was established the average homozygosity of the inbreds was 98.5 %, the average gene diversity index was 0.1723, and age genetic distance was 0.4975. It was found the allelic state of SNP markers of the BDI-III panel-270C and BDI-III-292A, which is characteristic of maize inbreds resistant to head smut, as well as BDI-III-270A and BDI-III-292G for maize inbreds sensitive to head smut. The dendrogram of genetic relationships studied maize inbreds was drawn.nConclusions. nThe presence of statistically significant associations the allelic state of two SNP markers and the phenotypic manifestation of the maize resistance to head smut established. The random nature of the location of maize inbreds sensitive to head smut on the dendrogram genetic relationships was revealed. In the future, the identified single-nucleotide polymorphism markers, were associated with head smut resistance, will be verified on a larger number of maize genotypes of origin to confirm the possibility of their practical application.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agricultural Science and Practice

ISSN

2312-3370

e-ISSN

2312-3389

Svazek periodika

12

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

UA - Ukrajina

Počet stran výsledku

10

Strana od-do

63-72

Kód UT WoS článku

001504397200005

EID výsledku v databázi Scopus

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