Meta-Topolin as an Aromatic Cytokinin for In Vitro Propagation of Thymus vulgaris L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00645459" target="_blank" >RIV/61389030:_____/25:00645459 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/25:73634824

Výsledek na webu

<a href="https://doi.org/10.3390/plants14233567" target="_blank" >https://doi.org/10.3390/plants14233567</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants14233567" target="_blank" >10.3390/plants14233567</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Meta-Topolin as an Aromatic Cytokinin for In Vitro Propagation of Thymus vulgaris L.

Popis výsledku v původním jazyce

Effective in vitro propagation of medicinal and aromatic plants such as Thymus vulgaris is often limited by the choice of optimal cytokinin sources, which are critical for successful shoot proliferation and overall regeneration. Since their discovery, topolins have been recognized as alternatives to traditional cytokinins (CKs) such as benzyladenine (BA) and kinetin (Kin) in plant tissue culture (PTC). This study investigated the influence of three aromatic CKs (meta-topolin (mT), BA, and Kin), on shoot proliferation of thyme, with the goal of improving current PTC protocols. Of all the tested treatments, the highest shoot proliferation (7.25 +/- 0.72 shoots per nodal explant) was observed in the treatment with 1 mu M mT, superior to BA and Kin. Increasing mT and BA concentrations from 1 to 15 mu M had an inverse effect on shoot production. The addition of indole-3-butyric acid (IBA) at concentrations ranging from 1.0 to 5.0 mu M in combination with 0.5 and 1 mu M mT did not increase the mean shoot numbers per explant. Regenerated shoots exhibited a strong propensity for root development even in the absence of plant growth regulators with 100% survival ex vitro. This study demonstrated that mT is an effective, sustainable and cost-effective alternative to traditional CKs for in vitro propagation of T. vulgaris, achieving an improved shoot proliferation with 1 mu M mT application.

Název v anglickém jazyce

Meta-Topolin as an Aromatic Cytokinin for In Vitro Propagation of Thymus vulgaris L.

Popis výsledku anglicky

Effective in vitro propagation of medicinal and aromatic plants such as Thymus vulgaris is often limited by the choice of optimal cytokinin sources, which are critical for successful shoot proliferation and overall regeneration. Since their discovery, topolins have been recognized as alternatives to traditional cytokinins (CKs) such as benzyladenine (BA) and kinetin (Kin) in plant tissue culture (PTC). This study investigated the influence of three aromatic CKs (meta-topolin (mT), BA, and Kin), on shoot proliferation of thyme, with the goal of improving current PTC protocols. Of all the tested treatments, the highest shoot proliferation (7.25 +/- 0.72 shoots per nodal explant) was observed in the treatment with 1 mu M mT, superior to BA and Kin. Increasing mT and BA concentrations from 1 to 15 mu M had an inverse effect on shoot production. The addition of indole-3-butyric acid (IBA) at concentrations ranging from 1.0 to 5.0 mu M in combination with 0.5 and 1 mu M mT did not increase the mean shoot numbers per explant. Regenerated shoots exhibited a strong propensity for root development even in the absence of plant growth regulators with 100% survival ex vitro. This study demonstrated that mT is an effective, sustainable and cost-effective alternative to traditional CKs for in vitro propagation of T. vulgaris, achieving an improved shoot proliferation with 1 mu M mT application.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plants

ISSN

2223-7747

e-ISSN

2223-7747

Svazek periodika

14

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

3567

Kód UT WoS článku

001634342700001

EID výsledku v databázi Scopus

2-s2.0-105024596376