Evaluation and comperison of therapeutic monitoring of whole-blood levels of cyclosporin A and its metabolites in renal transplantation by HPLC and RIA methods.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F01%3A00000003" target="_blank" >RIV/61988987:17110/01:00000003 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Evaluation and comperison of therapeutic monitoring of whole-blood levels of cyclosporin A and its metabolites in renal transplantation by HPLC and RIA methods.

Popis výsledku v původním jazyce

The aim of the work was to evaluate the possibilitty to estimate the level of cyclosporin A CyA metabolites as the difference of radioimmunoassay RIA non-specific and RIA specific methods: RIA specific method,RIA non-specific method,and high performanceliquid chromathograpdy method.Although values obtained by RIAsp correlated well those obtained by HPLC,the results of HPLC methods were lower by 8%. The values obtained by RIAns were 2,57 times higher than the values obtained by RIAsp.The combination ofboth the specific and non-specific methods for the determination of CyA presents an improved means for the TDM of CyA and CyA metabolites in renal transplant patients.Moreover,a combination of both methods can help to elucidate some unexpected events,such as the persistence of high cyclosporin blood levels.

Název v anglickém jazyce

Evaluation and comperison of therapeutic monitoring of whole-blood levels of cyclosporin A and its metabolites in renal transplantation by HPLC and RIA methods.

Popis výsledku anglicky

Evaluation and comperison of therapeutic monitoring of whole-blood levels of cyclosporin A and its metabolites in renal transplantation by HPLC and RIA methods. RIA specific method,RIA non-specific method,and high performance liquid chromathograpdy method.Although values obtained by RIAsp correlated well those obtained by HPLC,the results of HPLC methods were lower by 8%. The values obtained by RIAns were 2,57 times higher than the values obtained by RIAsp.The combination of both the specific and non-specific methods for the determination of CyA presents an improved means for the TDM of CyA and CyA metabolites in renal transplant patients.Moreover,a combination of both methods can help to elucidate some unexpected events,such as the persistence of highcyclosporin blood levels.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/OC%20B15.20" target="_blank" >OC B15.20: Plasmatická hladina cyklosporinu a jeho metabolitů a výskyt nežádoucích účinků (hypertenze, renální toxicita, rejekce) u transplantovaných pacientů. Pk/Pd modelování.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinica Chemica Acta 310

ISSN

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e-ISSN

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Svazek periodika

20

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

AS - Americká Samoa

Počet stran výsledku

226

Strana od-do

165-171

Kód UT WoS článku

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EID výsledku v databázi Scopus

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