Validation of Sparse Sampling Strateies to Estimate Cyclosporine A Area Under the Concentation-Time Curve Using Either a Specific Radioimmunoassay or Hign-Performance Liquid Chromography Method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F10%3AA110107H" target="_blank" >RIV/61988987:17110/10:A110107H - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Validation of Sparse Sampling Strateies to Estimate Cyclosporine A Area Under the Concentation-Time Curve Using Either a Specific Radioimmunoassay or Hign-Performance Liquid Chromography Method

Popis výsledku v původním jazyce

Area under the concentration-time curve (AUC) has been advocated as a better parameter to monitor cyclosporine A than trough concentrations. U to now, more thal 100 equations to estimate AUC using a limited sampling strategy have been published, but nothave been validated. Eight equations for AUC o-12h and two for AUC 0-8h were validated. Concetrations of cyclosporine A were analyzed by high-performance liquid chromatograhpy (HPLC) and a specific radioimmunoassay (RIA) method. Forty male renal transplant patients were included in the study. Blood samples were taken predose and at 0,5, 1, 1,5, 2, 3, 5, 8, and 12 hours after the morning dose when the patient was in steady state. The percentage prediction error (%pe) was used for an assessment of the performance of the equations. Mean %pe less than +/- and absolute %pe lees than 30% in 95% of predictions were considered to be acceptable. Other possibilities such as %pe less than 25%, 20ˇand 15ˇwere also tested.

Název v anglickém jazyce

Validation of Sparse Sampling Strateies to Estimate Cyclosporine A Area Under the Concentation-Time Curve Using Either a Specific Radioimmunoassay or Hign-Performance Liquid Chromography Method

Popis výsledku anglicky

Area under the concentration-time curve (AUC) has been advocated as a better parameter to monitor cyclosporine A than trough concentrations. U to now, more thal 100 equations to estimate AUC using a limited sampling strategy have been published, but nothave been validated. Eight equations for AUC o-12h and two for AUC 0-8h were validated. Concetrations of cyclosporine A were analyzed by high-performance liquid chromatograhpy (HPLC) and a specific radioimmunoassay (RIA) method. Forty male renal transplant patients were included in the study. Blood samples were taken predose and at 0,5, 1, 1,5, 2, 3, 5, 8, and 12 hours after the morning dose when the patient was in steady state. The percentage prediction error (%pe) was used for an assessment of the performance of the equations. Mean %pe less than +/- and absolute %pe lees than 30% in 95% of predictions were considered to be acceptable. Other possibilities such as %pe less than 25%, 20ˇand 15ˇwere also tested.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

THER DRUG MONIT

ISSN

0163-4356

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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