Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F17%3AA1801R36" target="_blank" >RIV/61988987:17110/17:A1801R36 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/65269705:_____/17:00066856 RIV/00843989:_____/17:E0105801
Výsledek na webu
<a href="http://dx.doi.org/10.1111/ejh.12779" target="_blank" >http://dx.doi.org/10.1111/ejh.12779</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ejh.12779" target="_blank" >10.1111/ejh.12779</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors
Popis výsledku v původním jazyce
Background: Aldehyde dehydrogenase (ALDH) is highly active in physiological stem cells as well as in tumor-initiating cells of some malignancies including multiple myeloma (MM). Finding higher activity of ALDH in some cell subsets in monoclonal gammopathies (MG) could identify potential source of myelomainitiating cells (MICs). Methods: Bone marrow of 12 MM, 9 monoclonal gammopathy of undetermined significance (MGUS), and 10 healthy donors (HD) were analyzed by flow cytometry. ALDH activity of B-cells and plasma cells (PC) was analyzed using Aldefluor. Results: Similar changes of ALDH activity were found during B-cell development in HD and MG. Decreasing of ALDH activity from immature to naive B-cells was found. In postgerminal stages, the activity started to increase, and in PCs, the ALDH activity was the same as in immature B-cells. Increased ALDH activity of all PC subsets compared to naive B-cells was found in MM as well as in HD, while in MGUS, only CD19(-)PCs have higher ALDH activity. In HD, ALDH activity was higher in CD19(+) PCs compared with MG. Conclusions: Our results indicate that changes of ALDH activity are the natural phenomenon in B-cell development; thus, high ALDH activity as a single marker is not appropriate for MICs identification.
Název v anglickém jazyce
Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors
Popis výsledku anglicky
Background: Aldehyde dehydrogenase (ALDH) is highly active in physiological stem cells as well as in tumor-initiating cells of some malignancies including multiple myeloma (MM). Finding higher activity of ALDH in some cell subsets in monoclonal gammopathies (MG) could identify potential source of myelomainitiating cells (MICs). Methods: Bone marrow of 12 MM, 9 monoclonal gammopathy of undetermined significance (MGUS), and 10 healthy donors (HD) were analyzed by flow cytometry. ALDH activity of B-cells and plasma cells (PC) was analyzed using Aldefluor. Results: Similar changes of ALDH activity were found during B-cell development in HD and MG. Decreasing of ALDH activity from immature to naive B-cells was found. In postgerminal stages, the activity started to increase, and in PCs, the ALDH activity was the same as in immature B-cells. Increased ALDH activity of all PC subsets compared to naive B-cells was found in MM as well as in HD, while in MGUS, only CD19(-)PCs have higher ALDH activity. In HD, ALDH activity was higher in CD19(+) PCs compared with MG. Conclusions: Our results indicate that changes of ALDH activity are the natural phenomenon in B-cell development; thus, high ALDH activity as a single marker is not appropriate for MICs identification.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30205 - Hematology
Návaznosti výsledku
Projekt
<a href="/cs/project/NV15-29508A" target="_blank" >NV15-29508A: Cirkulující nukleové kyseliny jako markery progrese mnohočetného myelomu</a><br>
Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
EUROPEAN JOURNAL OF HAEMATOLOGY
ISSN
0902-4441
e-ISSN
1600-0609
Svazek periodika
98
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
19-25
Kód UT WoS článku
000393166600002
EID výsledku v databázi Scopus
2-s2.0-84977142699